Anti-LGR5 Antibody (RACO0242)
- SKU:
- RACO0242
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | LGR5 Antibody |
| Product SKU: | RACO0242 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, FC |
| 推奨される希釈: | WB:1:500-1:5000, FC:1:20-1:200 |
| 反応性: | Human, Rat |
| 免疫原: | A synthesized peptide derived from human LGR5/GPR49 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. |
| シノニム: | Leucine-rich repeat-containing G-protein coupled receptor 5 (G-protein coupled receptor 49) (G-protein coupled receptor 67) (G-protein coupled receptor HG38), LGR5, GPR49 GPR67 |
 | Overlay histogram showing HepG2 cells stained with RACO0242 (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
 | Western Blot. Positive WB detected in SH-SY5Y whole cell lysate Rat brain tissue. All lanes LGR5 antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 100, 98, 93 kDa. Observed band size: 100 kDa. |
| UniProt Protein Function: | LGR5: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A marker of cycling, long-lived and multipotent adult stem cell populations. Target gene of Wnt signaling. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Intestinal Lgr5+ stem cells give rise throughout life to proliferation of cells in crypts, which migrate along the crypt-villus axis, differentiating into five main cell types. Wnt signaling plays a crucial role in the proliferation and differentiation of these crypt epithelial cells. Two isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 12q21.1 Cellular Component: integral to plasma membrane; plasma membrane; trans-Golgi network membrane Molecular Function:G-protein coupled receptor activity; protein binding; transmembrane receptor activity |
| NCBI Summary: | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| UniProt Code: | O75473 |
| NCBI GenInfo Identifier: | 21542118 |
| NCBI Gene ID: | 8549 |
| NCBI Accession: | O75473.1 |
| UniProt Secondary Accession: | O75473,Q4VAM0, Q4VAM2, Q9UP75, D8MCT0, |
| UniProt Related Accession: | O75473 |
| Molecular Weight: | 100kDa |
| NCBI Full Name: | Leucine-rich repeat-containing G-protein coupled receptor 5 |
| NCBI Synonym Full Names: | leucine rich repeat containing G protein-coupled receptor 5 |
| NCBI Official Symbol: | LGR5 |
| NCBI Official Synonym Symbols: | FEX; HG38; GPR49; GPR67; GRP49 |
| NCBI Protein Information: | leucine-rich repeat-containing G-protein coupled receptor 5 |
| UniProt Protein Name: | Leucine-rich repeat-containing G-protein coupled receptor 5 |
| UniProt Synonym Protein Names: | G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38 |
| Protein Family: | Leucine-rich repeat-containing G-protein coupled receptor |
| UniProt Gene Name: | LGR5 |