Anti-LEPRE1 Antibody (CAB10322)
- SKU:
- CAB10322
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-LEPRE1 Antibody |
| 抗体コード: | CAB10322 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 497-736 of human LEPRE1 (NP_071751.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | A375, NCI-H460, HT-29, HeLa, SKOV3 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 497-736 of human LEPRE1 (NP_071751.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GDGY RGQT SPHT PNEK FYGV TVFK ALKL GQEG KVPL QSAH LYYN VTEK VRRI MESY FRLD TPLY FSYS HLVC RTAI EEVQ AERK DDSH PVHV DNCI LNAE TLVC VKEP PAYT FRDY SAIL YLNG DFDG GNFY FTEL DAKT VTAE VQPQ CGRA VGFS SGTE NPHG VKAV TRGQ RCAI ALWF TLDP RHSE RDRV QADD LVKM LFSP EEMD LSQE QPLD AQQG PPEP AQES LSGS ESKP KDEL |
| 遺伝子ID: | 64175 |
| Uniprot: | Q32P28 |
| セルラーロケーション: | Endoplasmic reticulum, Secreted, extracellular matrix, extracellular space |
| 計算された分子量: | 41kDa/78kDa/83kDa/90kDa |
| 観察された分子量: | 100kDa |
| 同義語: | P3H1, GROS1, LEPRE1, OI8 |
| バックグラウンド: | This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. |
| UniProt Protein Function: | LEPRE1: Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro- Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts. Defects in LEPRE1 are the cause of osteogenesis imperfecta type 8 (OI8). A connective tissue disorder characterized by disproportionate short stature, severe osteoporosis, shortening of the long bones, white sclerae, a round face and a short barrel-shaped chest. Belongs to the leprecan family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.14.11.7; Secreted, signal peptide; Oxidoreductase; Cell development/differentiation; Secreted Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; membrane Molecular Function:collagen binding; procollagen-proline 3-dioxygenase activity; protein complex binding Biological Process: collagen metabolic process; protein amino acid hydroxylation; protein folding; protein stabilization; regulation of protein secretion Disease: Osteogenesis Imperfecta, Type Viii |
| NCBI Summary: | This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q32P28 |
| NCBI GenInfo Identifier: | 109892809 |
| NCBI Gene ID: | 64175 |
| NCBI Accession: | Q32P28.2 |
| UniProt Secondary Accession: | Q32P28,Q7KZR4, Q96BR8, Q96SK8, Q96SL5, Q96SN3, Q9H6K3 Q9HC86, Q9HC87, |
| UniProt Related Accession: | Q32P28 |
| Molecular Weight: | 78,922 Da |
| NCBI Full Name: | Prolyl 3-hydroxylase 1 |
| NCBI Synonym Full Names: | prolyl 3-hydroxylase 1 |
| NCBI Official Symbol: | P3H1 |
| NCBI Official Synonym Symbols: | OI8; GROS1; LEPRE1 |
| NCBI Protein Information: | prolyl 3-hydroxylase 1 |
| UniProt Protein Name: | Prolyl 3-hydroxylase 1 |
| UniProt Synonym Protein Names: | Growth suppressor 1 |
| UniProt Gene Name: | P3H1 |
| UniProt Entry Name: | P3H1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LEPRE1 antibody (CAB10322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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