Anti-LC3B 抗体 (CAB7198)

Anti-LC3B Antibody (CAB7198)[KO Validated]

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SKU:

CAB7198

Product type:

Antibody

Reactivity:

Human

Reactivity:

Mouse

Reactivity:

Rat

Host Species:

Rabbit

Isotype:

IgG

Antibody Type:

Polyclonal Antibody

Research Area:

Autophagy

Description

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抗体名:	Anti-LC3B Antibody [KO Validated]
抗体コード:	CAB7198
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	A synthetic peptide of human LC3B

免疫原:	A synthetic peptide of human LC3B
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	Email for sequence
遺伝子ID:	81631
Uniprot:	Q9GZQ8
セルラーロケーション:	Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
計算された分子量:	14kDa
観察された分子量:	14kDa, 16kDa

同義語:	LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A/LC3B
バックグラウンド:	The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

NCBI Summary:	The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9GZQ8
NCBI GenInfo Identifier:	17433141
NCBI Gene ID:	81631
NCBI Accession:	Q9GZQ8.3
UniProt Related Accession:	Q9GZQ8
Molecular Weight:
NCBI Full Name:	Microtubule-associated proteins 1A/1B light chain 3B
NCBI Synonym Full Names:	microtubule associated protein 1 light chain 3 beta
NCBI Official Symbol:	MAP1LC3B
NCBI Official Synonym Symbols:	LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
NCBI Protein Information:	microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name:	Microtubule-associated proteins 1A/1B light chain 3B
UniProt Synonym Protein Names:	Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
UniProt Gene Name:	MAP1LC3B
UniProt Entry Name:	MLP3B_HUMAN

	Western blot analysis of extracts of various cell lines, using LC3B antibody (CAB7198) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 uM) for 20 hours. Hela cells were treated by Chloroquine (50 uM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of pig liver, using LC3B antibody (CAB7198). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (CAB7198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody (CAB7198) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using LC3B antibody (CAB7198) at dilution of 1:100 (40x lens).