Anti-LC3B Antibody (CAB11282)[KO Validated]

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SKU:
CAB11282
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
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Description

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抗体名:Anti-LC3B Antibody [KO Validated]
抗体コード:CAB11282
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IF
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:A synthetic peptide of human LC3B
申し込み:WB IF
推奨希釈:WB 1:500 - 1:2000 IF 1:50 - 1:200
反応性:Human, Mouse, Rat
ポジティブサンプル:C6, NIH/3T3, Mouse brain, Rat brain
免疫原:A synthetic peptide of human LC3B
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:Email for sequence
遺伝子ID:81631
Uniprot:Q9GZQ8
セルラーロケーション:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
計算された分子量:14kDa
観察された分子量:16kDa
同義語:LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A/LC3B
バックグラウンド: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
NCBI Summary:The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
UniProt Code:Q9GZQ8
NCBI GenInfo Identifier:17433141
NCBI Gene ID:81631
NCBI Accession:Q9GZQ8.3
UniProt Related Accession:Q9GZQ8
Molecular Weight:
NCBI Full Name:Microtubule-associated proteins 1A/1B light chain 3B
NCBI Synonym Full Names:microtubule associated protein 1 light chain 3 beta
NCBI Official Symbol:MAP1LC3B  
NCBI Official Synonym Symbols:LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3  
NCBI Protein Information:microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name:Microtubule-associated proteins 1A/1B light chain 3B
UniProt Synonym Protein Names:Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
UniProt Gene Name:MAP1LC3B  
UniProt Entry Name:MLP3B_HUMAN
Anti-LC3B Antibody [KO Validated] (CAB11282)Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (CAB11282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-LC3B Antibody [KO Validated] (CAB11282)Western blot analysis of extracts of various cell lines, using LC3B antibody (CAB11282) at 1:1000 dilution. Both C6 cells and NIH/3T3 cells were treated by Chloroquine (50 uM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Anti-LC3B Antibody [KO Validated] (CAB11282)Immunofluorescence analysis of NIH-3T3 treated by chloroquine and NIH-3T3 cells using [KO Validated] LC3B Rabbit pAb (CAB11282) at dilution of 100 (40x lens).NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.
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