Anti-LC3A/LC3B Antibody (CAB5618)
- SKU:
- CAB5618
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| 抗体名: | Anti-LC3A/LC3B Antibody |
| 抗体コード: | CAB5618 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-121 of human LC3A (NP_115903.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, SH-SY5Y, A375, Mouse brain, Rat brain, NIH/3T3, C6 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-121 of human LC3A (NP_115903.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPSD RPFK QRRS FADR CKEV QQIR DQHP SKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELV KIIR RRLQ LNPT QAFF LLVN QHSM VSVS TPIA DIYE QEKD EDGF LYMV YASQ ETFG F |
| 遺伝子ID: | 84557/81631 |
| Uniprot: | Q9GZQ8/Q9H492 |
| セルラーロケーション: | Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton |
| 計算された分子量: | 14kDa |
| 観察された分子量: | 14kDa/16kDa |
| 同義語: | MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, MAP1LC3A, ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, LC3A/LC3B |
| バックグラウンド: | MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. |
| NCBI Summary: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9GZQ8 |
| NCBI GenInfo Identifier: | 17433141 |
| NCBI Gene ID: | 81631 |
| NCBI Accession: | Q9GZQ8.3 |
| UniProt Related Accession: | Q9GZQ8 |
| Molecular Weight: | |
| NCBI Full Name: | Microtubule-associated proteins 1A/1B light chain 3B |
| NCBI Synonym Full Names: | microtubule associated protein 1 light chain 3 beta |
| NCBI Official Symbol: | MAP1LC3B |
| NCBI Official Synonym Symbols: | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3 |
| NCBI Protein Information: | microtubule-associated proteins 1A/1B light chain 3B |
| UniProt Protein Name: | Microtubule-associated proteins 1A/1B light chain 3B |
| UniProt Synonym Protein Names: | Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta |
| UniProt Gene Name: | MAP1LC3B |
| UniProt Entry Name: | MLP3B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (CAB5618) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (CAB5618) at 1:500 dilution.293T cells were treated by Chloroquine (50 uM) at 37'C for 20 hours.NIH/3T3 cells were treated by Chloroquine (50 uM) at 37'C for 20 hours.C6 cells were treated by Chloroquine (50 uM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
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