Anti-KRT14 抗体 (CAB15069)

抗体名:	Anti-KRT14 Antibody
抗体コード:	CAB15069
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human KRT14 (NP_000517.2).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
反応性:	Human, Mouse, Rat
ポジティブサンプル:	HepG2, Mouse brain, Rat brain

免疫原:	A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human KRT14 (NP_000517.2).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	ILLD VKTR LEQE IATY RRLL EGED AHLS SSQF SSGS QSSR DVTS SSRQ IRTK VMDV HDGK VVST HEQV LRTK N
遺伝子ID:	3861
Uniprot:	P02533
セルラーロケーション:	Cytoplasm, Nucleus
計算された分子量:	51kDa
観察された分子量:	52KDa

同義語:

CK14, EBS3, EBS4, K14, NFJ, Cytokeratin 14, KRT14

バックグラウンド:

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

UniProt Protein Function:	K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: cytoplasm; keratin filament; intermediate filament; cytosol; nucleus Molecular Function:protein binding; structural constituent of cytoskeleton Biological Process: epidermis development; hemidesmosome assembly; epithelial cell differentiation; response to zinc ion; response to ionizing radiation; intermediate filament bundle assembly; hair cycle; aging Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type
NCBI Summary:	This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
UniProt Code:	P02533
NCBI GenInfo Identifier:	229463044
NCBI Gene ID:	3861
NCBI Accession:	P02533.4
UniProt Secondary Accession:	P02533,P08779,
UniProt Related Accession:	P02533
Molecular Weight:	~ 52kDa
NCBI Full Name:	Keratin, type I cytoskeletal 14
NCBI Synonym Full Names:	keratin 14
NCBI Official Symbol:	KRT14
NCBI Official Synonym Symbols:	K14; NFJ; CK14; EBS3; EBS4
NCBI Protein Information:	keratin, type I cytoskeletal 14
UniProt Protein Name:	Keratin, type I cytoskeletal 14
UniProt Synonym Protein Names:	Cytokeratin-14; CK-14; Keratin-14; K14
Protein Family:	Putative outer capsid protein
UniProt Gene Name:	KRT14
UniProt Entry Name:	K1C14_HUMAN

	Western blot analysis of extracts of various cell lines, using KRT14 Rabbit pAb (CAB15069) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Rat skin using KRT14 Rabbit pAb (CAB15069) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse skin using KRT14 Rabbit pAb (CAB15069) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of Rat skin cells using KRT14 antibody (CAB15069) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Human skin cells using KRT14 antibody (CAB15069) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-Cytokeratin 14 Antibody (CAB19039)

KRT14 Antibody (PACO01887)

KRT14 Antibody (PACO04179)

KRT14 Antibody (PACO10161)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)