Anti-KRT12 Antibody (CAB14746)
- SKU:
- CAB14746
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-KRT12 Antibody |
| 抗体コード: | CAB14746 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 295-494 of human KRT12 (NP_000214.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse eye, Rat eye |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 295-494 of human KRT12 (NP_000214.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | TRLL NDMR AQYE TIAE QNRK DAEA WFIE KSGE LRKE ISTN TEQL QSSK SEVT DLRR AFQN LEIE LQSQ LAMK KSLE DSLA EAEG DYCA QLSQ VQQL ISNL EAQL LQVR ADAE RQNV DHQR LLNV KARL ELEI ETYR RLLD GEAQ GDGL EESL FVTD SKSQ AQST DSSK DPTK TRKI KTVV QEMV NGEV VSSQ VQEI EELM |
| 遺伝子ID: | 3859 |
| Uniprot: | Q99456 |
| セルラーロケーション: | |
| 計算された分子量: | 53kDa |
| 観察された分子量: | 56kDa |
| 同義語: | KRT12, K12 |
| バックグラウンド: | KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. |
| UniProt Protein Function: | K12: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity. Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD); also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. Belongs to the intermediate filament family. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 17q12 Biological Process: visual perception Disease: Corneal Dystrophy, Meesmann |
| NCBI Summary: | KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99456 |
| NCBI GenInfo Identifier: | 2497269 |
| NCBI Gene ID: | 3859 |
| NCBI Accession: | Q99456.1 |
| UniProt Secondary Accession: | Q99456,B2R9E0, |
| UniProt Related Accession: | Q99456 |
| Molecular Weight: | 53,511 Da |
| NCBI Full Name: | Keratin, type I cytoskeletal 12 |
| NCBI Synonym Full Names: | keratin 12 |
| NCBI Official Symbol: | KRT12 |
| NCBI Official Synonym Symbols: | K12 |
| NCBI Protein Information: | keratin, type I cytoskeletal 12 |
| UniProt Protein Name: | Keratin, type I cytoskeletal 12 |
| UniProt Synonym Protein Names: | Cytokeratin-12; CK-12; Keratin-12; K12 |
| Protein Family: | Keratin |
| UniProt Gene Name: | KRT12 |
| UniProt Entry Name: | K1C12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KRT12 antibody (CAB14746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
View AllClose