Anti-KNTC1 Antibody (CAB13064)
- SKU:
- CAB13064
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-KNTC1 Antibody |
| 抗体コード: | CAB13064 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1100 of human KNTC1 (NP_055523.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1100 of human KNTC1 (NP_055523.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | IDLI DREQ GEDC LLLL KSLP PAEA EKTA ERVI IWAR LALQ EEPD HSKE GKAW RMSV AKTS VDIL KILC DIQK DNLQ KKDE CEEM LKLF KEVA SLQE NFEV FLSF EDYS NSSL VADL REQH IKAH EVAQ AKHK PGST PEPI AAEV RSPS MESK LHRQ ALAL QMSK QELE AELT LRAL KDGN IKTA LKKC SDLF KYHC NADT G |
| 遺伝子ID: | 9735 |
| Uniprot: | P50748 |
| セルラーロケーション: | Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore, spindle |
| 計算された分子量: | 51kDa/250kDa |
| 観察された分子量: | 250kDa |
| 同義語: | KNTC1, ROD |
| バックグラウンド: | This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. |
| UniProt Protein Function: | KNTC1: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: actin cytoskeleton; cytosol; kinetochore microtubule; nucleus; plasma membrane; spindle pole Molecular Function:protein binding Biological Process: cell division; mitotic cell cycle checkpoint; protein complex assembly; regulation of exit from mitosis; sister chromatid cohesion |
| NCBI Summary: | This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50748 |
| NCBI GenInfo Identifier: | 1519244775 |
| NCBI Gene ID: | 9735 |
| NCBI Accession: | NM_014708.6 |
| UniProt Related Accession: | P50748 |
| Molecular Weight: | |
| NCBI Full Name: | Homo sapiens kinetochore associated 1 (KNTC1), mRNA |
| NCBI Synonym Full Names: | kinetochore associated 1 |
| NCBI Official Symbol: | KNTC1 |
| NCBI Official Synonym Symbols: | ROD |
| NCBI Protein Information: | kinetochore-associated protein 1 |
| UniProt Protein Name: | Kinetochore-associated protein 1 |
| UniProt Synonym Protein Names: | Rough deal homolog; HsROD; Rod; hRod |
| Protein Family: | Kinetochore-associated protein |
| UniProt Gene Name: | KNTC1 |
 | Western blot analysis of extracts of 293T cells, using KNTC1 antibody (CAB13064) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |