Ki67 protein is expressed by dividing cells but is rarely found in resting cells which makes it a reliable marker for cell proliferation. The presence of Ki67 can be used to infer how well cancer therapy has worked on cancer cells and the rate of tumor growth. Proliferating cancer cells are sensitive to chemotherapy, while non proliferating cells are generally not affected by such treatments. Ki67 is often used as a marker in assays to test various chemotherapies on tumors that display high proliferation (such as neuroblastomas).
MKI67, KIA, MIB-, MIB-1, PPP1R105, marker of proliferation Ki-67, Ki67
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This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
Western blot analysis of extracts of various cell lines, using Ki67 Rabbit pAb (CAB2094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using Ki67 antibody (CAB2094) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using Ki67 antibody (CAB2094) at dilution of 1:100. Blue: DAPI for nuclear staining.
