Anti-Kdm6b Antibody (CAB12763)
- SKU:
- CAB12763
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Frequently bought together:
Description
| 抗体名: | Anti-Kdm6b Antibody |
| 抗体コード: | CAB12763 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 265-450 of mouse Kdm6b (NP_001017426.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | SH-SY5Y, K-562, mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 265-450 of mouse Kdm6b (NP_001017426.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | PPLP GLAI SPPF QLTK PGLW NTLH GDAW GPER KGSA PPER QEQR HSMP HSYP YPAP AYSA HPPS HRLV PNTP LGPG PRPP GAES HGCL PATR PPGS DLRE SRVQ RSRM DSSV SPAA STAC VPYA PSRP PGLP GTSS SSSS SSSS NNTG LRGV EPSP GIPG ADHY QNPA LEIS PHQA RLGP SAHS SR |
| 遺伝子ID: | 216850 |
| Uniprot: | |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 176kDa |
| 観察された分子量: | 170kDa |
| 同義語: | KDM6B, JMJD3, Kdm6b |
| バックグラウンド: |
 | Western blot analysis of extracts of various cell lines, using Kdm6b antibody (CAB12763) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Western blot analysis of extracts of mouse brain, using KDM6B antibody (CAB12763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of NIH/3T3 cells using KDM6B antibody (CAB12763) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using KDM6B antibody (CAB12763) at dilution of 1:100. Blue: DAPI for nuclear staining. |