Anti-KDM5A Antibody (CAB7238)
- SKU:
- CAB7238
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-KDM5A Antibody |
| 抗体コード: | CAB7238 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human KDM5A |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | Jurkat, HeLa, MCF7 |
| 免疫原: | Recombinant protein of human KDM5A |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 5927 |
| Uniprot: | P29375 |
| セルラーロケーション: | Nucleus, nucleolus |
| 計算された分子量: | 186kDa/192kDa |
| 観察された分子量: | 192kDa |
| 同義語: | KDM5A, RBBP-2, RBBP2, RBP2 |
| バックグラウンド: | This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. |
| UniProt Protein Function: | JARID1A: Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Interacts with SUZ12; the interaction is direct. Interacts with the viral protein-binding domain of RB1. Interacts with ESR1, MYC, MYCN and LMO2. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nucleolus; Oxidoreductase; Transcription factor; EC 1.14.11.-; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 12p11 Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex Molecular Function:protein binding; DNA binding; zinc ion binding; transcription coactivator activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; chromatin binding; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; positive regulation of transcription, DNA-dependent; multicellular organismal development; circadian regulation of gene expression; chromatin modification |
| NCBI Summary: | This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P29375 |
| NCBI GenInfo Identifier: | 215274124 |
| NCBI Gene ID: | 5927 |
| NCBI Accession: | P29375.3 |
| UniProt Secondary Accession: | P29375,Q4LE72, Q86XZ1, A8MV76, |
| UniProt Related Accession: | P29375 |
| Molecular Weight: | 1690 |
| NCBI Full Name: | Lysine-specific demethylase 5A |
| NCBI Synonym Full Names: | lysine (K)-specific demethylase 5A |
| NCBI Official Symbol: | KDM5A |
| NCBI Official Synonym Symbols: | RBP2; RBBP2; RBBP-2 |
| NCBI Protein Information: | lysine-specific demethylase 5A; histone demethylase JARID1A; retinoblastoma binding protein 2; jumonji/ARID domain-containing protein 1A; Jumonji, AT rich interactive domain 1A (RBP2-like) |
| UniProt Protein Name: | Lysine-specific demethylase 5A |
| UniProt Synonym Protein Names: | Histone demethylase JARID1A; Jumonji/ARID domain-containing protein 1A; Retinoblastoma-binding protein 2; RBBP-2 |
| Protein Family: | Lysine-specific demethylase |
| UniProt Gene Name: | KDM5A |
| UniProt Entry Name: | KDM5A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KDM5A antibody (CAB7238) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
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