Anti-JNK2 Antibody (CAB1251)[KO Validated]

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SKU:
CAB1251
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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Description

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抗体名:Anti-JNK2 Antibody [KO Validated]
抗体コード:CAB1251
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC IF
反応性:Human, Mouse
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human JNK2 (NP_002743.3).
申し込み:WB IHC IF
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:Human, Mouse
ポジティブサンプル:MCF-7, DU145, THP-1, Mouse liver
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human JNK2 (NP_002743.3).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MSDS KCDS QFYS VQVA DSTF TVLK RYQQ LKPI GSGA QGIV CAAF DTVL GINV AVKK LSRP FQNQ THAK RAYR ELVL LKCV NHKN IISL LNVF TPQK TLEE FQDV YLVM ELMD ANLC QVIH MELD HERM SYLL YQML CGIK HLHS AGII HRDL KPSN IVVK SDCT LKIL DFGL ARTA CTNF MMTP YVVT RYYR APEV ILGM GYKE NVDI WSVG CIMG ELVK GCVI FQGT DHID QWNK VIEQ LGTP SAEF MKKL QPTV RNYV ENRP KYPG IKFE ELFP DWIF PSES ERDK IKTS QARD LLSK MLVI DPDK RISV DEAL RHPY ITVW YDPA EAEA PPPQ IYDA QLEE REHA IEEW KELI YKEV MDWE ERSK NGVV KDQP SDAA VSSN ATPS QSSS INDI SSMS TEQT LASD TDSS LDAS TGPL EGCR
遺伝子ID:5601
Uniprot:P45984
セルラーロケーション:Cytoplasm, Nucleus
計算された分子量:27kDa/44kDa/48kDa
観察された分子量:54kDa
同義語:MAPK9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK
バックグラウンド:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
UniProt Protein Function:JNK2: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses including UV radiation. Phosphorylates specific transcription factors such as c-Jun and ATF2, mediating immediate-early gene expression. Closely related to JNK1. Both are involved in UV radiation induced apoptosis. Blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells in the mouse. Four alternatively-spliced isoforms have been described.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; Protein kinase, CMGC; Kinase, protein; CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nucleoplasm; mitochondrion; cytosol

Molecular Function:protein binding; mitogen-activated protein kinase kinase kinase binding; caspase activator activity; JUN kinase activity; transcription factor binding; ATP binding

Biological Process: central nervous system development; positive regulation of nitric oxide biosynthetic process; regulation of protein ubiquitination; response to toxin; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; rhythmic process; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; regulation of JNK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; response to stress; JNK cascade; protein targeting to mitochondrion; toll-like receptor 4 signaling pathway; neurite development; positive regulation of prostaglandin biosynthetic process; caspase activation; response to drug; release of cytochrome c from mitochondria; MyD88-independent toll-like receptor signaling pathway; regulation of circadian rhythm; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; JUN phosphorylation; MyD88-dependent toll-like receptor signaling pathway; response to cadmium ion; response to mechanical stimulus; positive regulation of prostaglandin secretion; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; positive regulation of protein amino acid phosphorylation; response to amine stimulus; positive regulation of nitric-oxide synthase biosynthetic process

NCBI Summary:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]
UniProt Code:P45984
NCBI GenInfo Identifier:85700366
NCBI Gene ID:5601
NCBI Accession:P45984.2
UniProt Secondary Accession:P45984,Q15708, Q15710, Q15711, Q8N5C5, A8K0S3, B5BU66 B5M0B4, D3DWQ8, D3DWQ9,
UniProt Related Accession:P45984
Molecular Weight:27,334 Da
NCBI Full Name:Mitogen-activated protein kinase 9
NCBI Synonym Full Names:mitogen-activated protein kinase 9
NCBI Official Symbol:MAPK9  
NCBI Official Synonym Symbols:JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA  
NCBI Protein Information:mitogen-activated protein kinase 9; MAPK 9; Jun kinase; MAP kinase 9; c-Jun kinase 2; c-Jun N-terminal kinase 2; stress-activated protein kinase 1a; stress-activated protein kinase JNK2
UniProt Protein Name:Mitogen-activated protein kinase 9
UniProt Synonym Protein Names:JNK-55; Stress-activated protein kinase 1a; SAPK1a; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2
UniProt Gene Name:MAPK9  
UniProt Entry Name:MK09_HUMAN
Anti-JNK2 Antibody [KO Validated] (CAB1251)Western blot analysis of extracts of various cell lines, using JNK2 antibody (CAB1251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-JNK2 Antibody [KO Validated] (CAB1251)Western blot analysis of extracts from normal (control) and JNK2 knockout (KO) 293T cells, using JNK2 antibody (CAB1251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-JNK2 Antibody [KO Validated] (CAB1251)Immunohistochemistry of paraffin-embedded human breast cancer using JNK2 antibody (CAB1251) at dilution of 1:100 (40x lens).
Anti-JNK2 Antibody [KO Validated] (CAB1251)Immunohistochemistry of paraffin-embedded mouse brain using JNK2 antibody (CAB1251) at dilution of 1:100 (40x lens).
Anti-JNK2 Antibody [KO Validated] (CAB1251)Immunofluorescence analysis of U2OS cells using JNK2 antibody (CAB1251). Blue: DAPI for nuclear staining.
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