Anti-ITGA4 Antibody (RACO0298)
- SKU:
- RACO0298
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
商品名: | ITGA4 Antibody |
Product SKU: | RACO0298 |
サイズ: | 50ul |
宿主種: | Homo sapiens (Human) |
申し込み: | ELISA, WB |
推奨される希釈: | WB:1:500-1:5000 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human Integrin alpha 4 |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). |
シノニム: | Integrin alpha-4 (CD49 antigen-like family member D) (Integrin alpha-IV) (VLA-4 subunit alpha) (CD antigen CD49d), ITGA4, CD49D |
Western Blot. Positive WB detected in Jurkat whole cell lysate All lanes ITGA4 antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 115, 26 kDa. Observed band size: 140 kDa. |
UniProt Protein Function: | ITGA4: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Belongs to the integrin alpha chain family. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Receptor, misc. Chromosomal Location of Human Ortholog: 2q31.3 Cellular Component: focal adhesion; cell surface; membrane; plasma membrane; intercellular junction; external side of plasma membrane Molecular Function:protein binding; fibronectin binding; metal ion binding; cell adhesion molecule binding Biological Process: integrin-mediated signaling pathway; regulation of immune response; extracellular matrix organization and biogenesis; heart development; cell-matrix adhesion; receptor clustering; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; B cell differentiation; blood vessel remodeling; blood coagulation; leukocyte tethering or rolling; leukocyte migration |
NCBI Summary: | The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] |
UniProt Code: | P13612 |
NCBI GenInfo Identifier: | 311033436 |
NCBI Gene ID: | 3676 |
NCBI Accession: | P13612.3 |
UniProt Related Accession: | P13612 |
Molecular Weight: | |
NCBI Full Name: | Integrin alpha-4 |
NCBI Synonym Full Names: | integrin subunit alpha 4 |
NCBI Official Symbol: | ITGA4 |
NCBI Official Synonym Symbols: | IA4; CD49D |
NCBI Protein Information: | integrin alpha-4 |
UniProt Protein Name: | Integrin alpha-4 |
UniProt Synonym Protein Names: | CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD_antigen: CD49d |
Protein Family: | Integrin |
UniProt Gene Name: | ITGA4 |
UniProt Entry Name: | ITA4_HUMAN |