Anti-ISG15 Antibody (CAB1182)
- SKU:
- CAB1182
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-ISG15 Antibody |
| 抗体コード: | CAB1182 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, PC-3, SW620, HCT116 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGWD LTVK MLAG NEFQ VSLS SSMS VSEL KAQI TQKI GVHA FQQR LAVH PSGV ALQD RVPL ASQG LGPG STVL LVVD KCDE PLSI LVRN NKGR SSTY EVRL TQTV AHLK QQVS GLEG VQDD LFWL TFEG KPLE DQLP LGEY GLKP LSTV FMNL RLRG GGTE PGGR S |
| 遺伝子ID: | 9636 |
| Uniprot: | P05161 |
| セルラーロケーション: | Cytoplasm, Secreted |
| 計算された分子量: | 17kDa |
| 観察された分子量: | 14kDa |
| 同義語: | ISG15, G1P2, IFI15, IMD38, IP17, UCRP, hUCRP |
| バックグラウンド: | The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. |
| UniProt Protein Function: | ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. |
| UniProt Protein Details: | Protein type:Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 1p36.33 Cellular Component: cytosol; extracellular region; nucleoplasm Molecular Function:protein binding Biological Process: bypass DNA synthesis; defense response to bacterium; defense response to virus; ISG15-protein conjugation; negative regulation of interferon type I production; negative regulation of protein ubiquitination; negative regulation of viral genome replication; positive regulation of bone mineralization; regulation of interferon-gamma production Disease: Immunodeficiency 38 |
| NCBI Summary: | The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012] |
| UniProt Code: | P05161 |
| NCBI GenInfo Identifier: | 52001470 |
| NCBI Gene ID: | 9636 |
| NCBI Accession: | P05161.5 |
| UniProt Secondary Accession: | P05161,Q5SVA4, Q7Z2G2, Q96GF0, |
| UniProt Related Accession: | P05161 |
| Molecular Weight: | 17,888 Da |
| NCBI Full Name: | Ubiquitin-like protein ISG15 |
| NCBI Synonym Full Names: | ISG15 ubiquitin-like modifier |
| NCBI Official Symbol: | ISG15 |
| NCBI Official Synonym Symbols: | G1P2; IP17; UCRP; IFI15; IMD38; hUCRP |
| NCBI Protein Information: | ubiquitin-like protein ISG15 |
| UniProt Protein Name: | Ubiquitin-like protein ISG15 |
| UniProt Synonym Protein Names: | Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP |
| Protein Family: | Ubiquitin-like protein |
| UniProt Gene Name: | ISG15 |
 | Western blot analysis of extracts of various cell lines, using ISG15 antibody (CAB1182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat ovary using ISG15 antibody (CAB1182) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human thyroid cancer using ISG15 antibody (CAB1182) at dilution of 1:100 (40x lens). |
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