Anti-IRF3 Antibody (CAB11373)
- SKU:
- CAB11373
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| 抗体名: | Anti-IRF3 Antibody |
| 抗体コード: | CAB11373 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | THP-1, HT-29 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGTP KPRI LPWL VSQL DLGQ LEGV AWVN KSRT RFRI PWKH GLRQ DAQQ EDFG IFQA WAEA TGAY VPGR DKPD LPTW KRNF RSAL NRKE GLRL AEDR SKDP HDPH KIYE FVNS GVGD FSQP DTSP DTNG GGST SDTQ EDIL DELL GNMV LAPL PDPG PPSL AVAP EPCP QPLR SPSL DNPT PFPN LGPS ENPL KRLL VPGE EWEF EVTA FYRG RQVF QQTI SCPE GLRL VGSE VGDR TLPG WPVT LPDP GMSL TDRG VMSY |
| 遺伝子ID: | 3661 |
| Uniprot: | Q14653 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 12kDa/16kDa/33kDa/47kDa/49kDa |
| 観察された分子量: | 54kDa |
| 同義語: | IIAE7, IRF3 |
| バックグラウンド: | This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 19q13.3-q13.4 Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; negative regulation of interferon-beta biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of defense response to virus by host; response to exogenous dsRNA; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon-beta production; innate immune response; interferon type I biosynthetic process; defense response to virus; toll-like receptor 4 signaling pathway; response to DNA damage stimulus; negative regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of cytokine secretion Disease: Herpes Simplex Encephalitis, Susceptibility To, 7 |
| NCBI Summary: | This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q14653 |
| NCBI GenInfo Identifier: | 2497442 |
| NCBI Gene ID: | 3661 |
| NCBI Accession: | Q14653.1 |
| UniProt Secondary Accession: | Q14653,Q5FBY1, Q5FBY2, Q5FBY4, Q7Z5G6, A8K7L2, B2RAZ3 |
| UniProt Related Accession: | Q14653 |
| Molecular Weight: | 12,330 Da |
| NCBI Full Name: | Interferon regulatory factor 3 |
| NCBI Synonym Full Names: | interferon regulatory factor 3 |
| NCBI Official Symbol: | IRF3 |
| NCBI Protein Information: | interferon regulatory factor 3 |
| UniProt Protein Name: | Interferon regulatory factor 3 |
| Protein Family: | Interferon regulatory factor |
| UniProt Gene Name: | IRF3 |
| UniProt Entry Name: | IRF3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IRF3 antibody (CAB11373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat lung using IRF3 antibody (CAB11373) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using IRF3 antibody (CAB11373) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using IRF3 antibody (CAB11373) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using IRF3 antibody (CAB11373) at dilution of 1:100 (40x lens). |