Anti-IBTK Antibody (CAB14866)
- SKU:
- CAB14866
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-IBTK Antibody |
| 抗体コード: | CAB14866 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1200 of human IBTK (NP_056340.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | Jurkat, HepG2 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1200 of human IBTK (NP_056340.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GLNM AALL EARS LDVL SDGV LKDL SEFY RKMI PAMD RRVI TPYQ DGPD ISYL EVED GDIF LKEE INME QNHS ETMF KKAK TKAK KKPR KRSD SSGG YNLS DIIQ SPSS TGLL KSGK TNSV ESLP ELLT SDSE GSYA GVGS PRDL QSPD FTTG FHSD KIEA KVKP YVNG TSPV YSRE DLKP WEKS PILK ISAP QPIP SNRI DTTS SASW VAGS FSPV SPPV VDLR TIME IEES RQKC GATP KSHL GKTV SHGV KLSQ KQRK MIAL TTKE NNSG MNSM ETVL FTPS KAPK PVNA WASS LHSV S |
| 遺伝子ID: | 25998 |
| Uniprot: | Q9P2D0 |
| セルラーロケーション: | Cytoplasm, Membrane, Nucleus, Peripheral membrane protein |
| 計算された分子量: | 26kDa/133kDa/150kDa |
| 観察された分子量: | 150kDa |
| 同義語: | IBTK, BTBD26, BTKI |
| バックグラウンド: | Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | IBTK: Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protein kinase, regulatory subunit Chromosomal Location of Human Ortholog: 6q14.1 Cellular Component: cytoplasm; nucleoplasm Molecular Function:protein kinase binding; protein tyrosine kinase inhibitor activity Biological Process: negative regulation of protein amino acid phosphorylation; release of sequestered calcium ion into cytosol |
| NCBI Summary: | Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q9P2D0 |
| NCBI GenInfo Identifier: | 134034141 |
| NCBI Gene ID: | 25998 |
| NCBI Accession: | Q9P2D0.3 |
| UniProt Secondary Accession: | Q9P2D0,Q2QKU2, Q2QKU3, Q2QKU4, Q5TFD7, Q5TFD9, Q8IUQ9 Q8IUY7, Q8TAI4, Q9HBI8, Q9Y3T8, |
| UniProt Related Accession: | Q9P2D0 |
| Molecular Weight: | 26,314 Da |
| NCBI Full Name: | Inhibitor of Bruton tyrosine kinase |
| NCBI Synonym Full Names: | inhibitor of Bruton tyrosine kinase |
| NCBI Official Symbol: | IBTK |
| NCBI Official Synonym Symbols: | BTKI; BTBD26 |
| NCBI Protein Information: | inhibitor of Bruton tyrosine kinase |
| UniProt Protein Name: | Inhibitor of Bruton tyrosine kinase |
| Protein Family: | Inhibitor of Bruton tyrosine kinase |
| UniProt Gene Name: | IBTK |
| UniProt Entry Name: | IBTK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IBTK antibody (CAB14866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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