Anti-HuR/ELAVL1 抗体 (CAB1608)

抗体名:	Anti-HuR / ELAVL1 Antibody [KO Validated]
抗体コード:	CAB1608
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR / ELAVL1 (NP_001410.2).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	U-251MG, HeLa, Jurkat, K-562, PC-3

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR / ELAVL1 (NP_001410.2).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MSNG YEDH MAED CRGD IGRT NLIV NYLP QNMT QDEL RSLF SSIG EVES AKLI RDKV AGHS LGYG FVNY VTAK DAER AINT LNGL RLQS KTIK VSYA RPSS EVIK DANL YISG LPRT MTQK DVED MFSR FGRI INSR VLVD QTTG LSRG VAFI RFDK RSEA EEAI TSFN GHKP PGSS EPIT VKFA ANPN QNKN VALL SQLY HSPA RRFG GPVH HQAQ RFRF SPMG VDHM SGLS GVNV PGNA SSGW CIFI YNLG QDAD EGIL WQMF GPFG AVTN VKVI RDFN TNKC KGFG FVTM TNYE EAAM AIAS LNGY RLGD KILQ VSFK TNKS HK
遺伝子ID:	1994
Uniprot:	Q15717
セルラーロケーション:	Cytoplasm, Nucleus
計算された分子量:	36kDa/38kDa
観察された分子量:	37kDa

同義語:

ELAV1, HUR, Hua, MelG, HuR / ELAVL1, ELAVL1

バックグラウンド:

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.

UniProt Protein Function:	HuR: Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ubiquitous. Belongs to the RRM elav family.
UniProt Protein Details:	Protein type:RNA-binding Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus Molecular Function:mRNA binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding Biological Process: positive regulation of translation; multicellular organismal development; mRNA stabilization; gene expression
NCBI Summary:	The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]
UniProt Code:	Q15717
NCBI GenInfo Identifier:	20981691
NCBI Gene ID:	1994
NCBI Accession:	Q15717.2
UniProt Related Accession:	Q15717
Molecular Weight:
NCBI Full Name:	ELAV-like protein 1
NCBI Synonym Full Names:	ELAV like RNA binding protein 1
NCBI Official Symbol:	ELAVL1
NCBI Official Synonym Symbols:	HUR; Hua; MelG; ELAV1
NCBI Protein Information:	ELAV-like protein 1
UniProt Protein Name:	ELAV-like protein 1
UniProt Synonym Protein Names:	Hu-antigen R; HuR
UniProt Gene Name:	ELAVL1
UniProt Entry Name:	ELAV1_HUMAN

	Western blot analysis of extracts of various cell lines, using HuR / ELAVL1 antibody (CAB1608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and HuR / ELAVL1 knockout (KO) 293T cells, using HuR / ELAVL1 antibody (CAB1608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse testis using HuR / ELAVL1 Antibody (CAB1608) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using HuR / ELAVL1 Antibody (CAB1608) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using HuR / ELAVL1 Antibody (CAB1608) at dilution of 1:200 (40x lens).

Antibodies

Anti-HuR / ELAVL1 Antibody (CAB6089)[KO Validated]

Anti-HuR / ELAVL1 Antibody [KO Validated] (CAB19622)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)