Anti-HNRNPC Antibody (RACO0191)
- SKU:
- RACO0191
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | HNRNPC Antibody |
| Product SKU: | RACO0191 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC, IF, FC, IP |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human HNRNPC |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). |
| シノニム: | Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2, HNRNPC, HNRPC |
 | IHC image of RACO0191 diluted at 1:66.35 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with RACO0191 at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in Hela whole cell lysate K562 whole cell lysate 293T whole cell lysate MCF-7 whole cell lysate Raji whole cell lysate Jurkat whole cell lysate HepG2 whole cell lysate All lanes hnRNP C1 + C2 antibody at 0.66µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 34, 33, 26, 28 KDa. Observed band size: 42 KDa. |
| UniProt Protein Function: | hnRNP C1/C2: a heterogeneous nuclear ribonucleoprotein. May play a role in ribonucleosome assembly by neutralizing basic proteins such as A and B core hnRNP. Contains 1 RNA recognition motif (RRM) domain. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Spliceosome; RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: cytosol; membrane; nuclear chromatin; nucleoplasm; nucleus; protein complex; spliceosome; telomerase holoenzyme complex Molecular Function:identical protein binding; mRNA 3'-UTR binding; nucleosomal DNA binding; nucleotide binding; poly(U) binding; protein binding; RNA binding Biological Process: ATP-dependent chromatin remodeling; cellular protein metabolic process; gene expression; negative regulation of telomere maintenance via telomerase; nuclear mRNA splicing, via spliceosome; osteoblast differentiation; post-translational protein modification; protein sumoylation; RNA splicing |
| NCBI Summary: | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07910 |
| NCBI GenInfo Identifier: | 108935845 |
| NCBI Gene ID: | 3183 |
| NCBI Accession: | P07910.4 |
| UniProt Secondary Accession: | P07910,P22628, Q53EX2, Q59FD3, Q5FWE8, Q86SF8, Q86U45 Q96HK7, Q96HM4, Q96IY5, D3DS19, D3DS22, |
| UniProt Related Accession: | P07910 |
| Molecular Weight: | 27,822 Da |
| NCBI Full Name: | Heterogeneous nuclear ribonucleoproteins C1/C2 |
| NCBI Synonym Full Names: | heterogeneous nuclear ribonucleoprotein C (C1/C2) |
| NCBI Official Symbol: | HNRNPC |
| NCBI Official Synonym Symbols: | C1; C2; HNRNP; HNRPC; SNRPC |
| NCBI Protein Information: | heterogeneous nuclear ribonucleoproteins C1/C2 |
| UniProt Protein Name: | Heterogeneous nuclear ribonucleoproteins C1/C2 |
| Protein Family: | Heterogeneous nuclear ribonucleoprotein |
| UniProt Gene Name: | HNRNPC |
| UniProt Entry Name: | HNRPC_HUMAN |