Anti-HNRNPA2B1 Antibody (CAB1162)[KO Validated]

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SKU:
CAB1162
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
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Description

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抗体名:Anti-HNRNPA2B1 Antibody [KO Validated]
抗体コード:CAB1162
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC IF
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA2B1 (NP_002128.1).
申し込み:WB IHC IF
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:Human, Mouse, Rat
ポジティブサンプル:HeLa, BxPC-3, PC-3, Mouse spleen
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA2B1 (NP_002128.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MERE KEQF RKLF IGGL SFET TEES LRNY YEQW GKLT DCVV MRDP ASKR SRGF GFVT FSSM AEVD AAMA ARPH SIDG RVVE PKRA VARE ESGK PGAH VTVK KLFV GGIK EDTE EHHL RDYF EEYG KIDT IEII TDRQ SGKK RGFG FVTF DDHD PVDK IVLQ KYHT INGH NAEV RKAL SRQE
遺伝子ID:3181
Uniprot:P22626
セルラーロケーション:Cytoplasm, Nucleus, nucleoplasm
計算された分子量:36kDa/37kDa
観察された分子量:37kDa
同義語:HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, IBMPFD2, RNPA2, SNRPB1, HNRNPA2B1
バックグラウンド:This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.
UniProt Protein Function:Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. Packaging plays a role in various processes such as transcription, pre-mRNA processing, RNA nuclear export, subcellular location, mRNA translation and stability of mature mRNAs (PubMed:19099192). Forms hnRNP particles with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm (PubMed:10567417). Specifically binds single-stranded telomeric DNA sequences, protecting telomeric DNA repeat against endonuclease digestion. Also binds other RNA molecules, such as primary miRNA (pri-miRNAs): acts as a nuclear 'reader' of the N6-methyladenosine (m6A) mark by specifically recognizing and binding a subset of nuclear m6A-containing pri-miRNAs. Binding to m6A-containing pri-miRNAs promotes pri-miRNA processing by enhancing binding of DGCR8 to pri-miRNA transcripts (PubMed:26321680). Involved in miRNA sorting into exosomes following sumoylation, possibly by binding (m6A)-containing pre-miRNAs (PubMed:24356509). Acts as a regulator of efficiency of mRNA splicing, possibly by binding to m6A-containing pre-mRNAs (PubMed:26321680).
NCBI Summary:This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:P22626
NCBI GenInfo Identifier:14043072
NCBI Gene ID:3181
NCBI Accession:NP_112533.1
UniProt Secondary Accession:P22626,P22627, Q9UC98, Q9UDJ2, A0A024RA27, A0A024RA61, A8K064
UniProt Related Accession:P22626
Molecular Weight:Predicted MW: 37kDa
NCBI Full Name:heterogeneous nuclear ribonucleoproteins A2/B1 isoform B1
NCBI Synonym Full Names:heterogeneous nuclear ribonucleoprotein A2/B1
NCBI Official Symbol:HNRNPA2B1  
NCBI Official Synonym Symbols:RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; IBMPFD2; HNRPA2B1  
NCBI Protein Information:heterogeneous nuclear ribonucleoproteins A2/B1
UniProt Protein Name:Heterogeneous nuclear ribonucleoproteins A2/B1
UniProt Gene Name:HNRNPA2B1  
Anti-HNRNPA2B1 Antibody [KO Validated] (CAB1162)Western blot analysis of extracts of various cell lines, using HNRNPA2B1 antibody (CAB1162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-HNRNPA2B1 Antibody [KO Validated] (CAB1162)Western blot analysis of extracts from normal (control) and HNRNPA2B1 knockout (KO) 293T cells, using HNRNPA2B1 antibody (CAB1162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-HNRNPA2B1 Antibody [KO Validated] (CAB1162)Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:100 (40x lens).
Anti-HNRNPA2B1 Antibody [KO Validated] (CAB1162)Immunohistochemistry of paraffin-embedded rat brain using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:100 (40x lens).
Anti-HNRNPA2B1 Antibody [KO Validated] (CAB1162)Immunohistochemistry of paraffin-embedded human colon using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:200 (40x lens).
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