Anti-HLA-DMB Antibody (CAB8384)
- SKU:
- CAB8384
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-HLA-DMB Antibody |
| 抗体コード: | CAB8384 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-218 of human HLA-DMB (NP_002109.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human |
| ポジティブサンプル: | Raji |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-218 of human HLA-DMB (NP_002109.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GGFV AHVE STCL LDDA GTPK DFTY CISF NKDL LTCW DPEE NKMA PCEF GVLN SLAN VLSQ HLNQ KDTL MQRL RNGL QNCA THTQ PFWG SLTN RTRP PSVQ VAKT TPFN TREP VMLA CYVW GFYP AEVT ITWR KNGK LVMP HSSA HKTA QPNG DWTY QTLS HLAL TPSY GDTY TCVV EHTG APEP ILRD WTPG LSPM QTLK |
| 遺伝子ID: | 3109 |
| Uniprot: | P28068 |
| セルラーロケーション: | Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein |
| 計算された分子量: | 28kDa |
| 観察された分子量: | 29kDa |
| 同義語: | HLA-DMB, D6S221E, RING7, major histocompatibility complex, class II, DM beta |
| バックグラウンド: | HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. |
| UniProt Protein Function: | HLA-DMB: Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Belongs to the MHC class II family. |
| UniProt Protein Details: | Protein type:Vesicle; Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: lysosomal membrane; late endosome membrane; integral to membrane; MHC class II protein complex Biological Process: peptide antigen assembly with MHC class II protein complex; antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of T cell proliferation; immune response; MHC class II protein complex assembly |
| NCBI Summary: | HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P28068 |
| NCBI GenInfo Identifier: | 133160 |
| NCBI Gene ID: | 3109 |
| NCBI Accession: | P28068.1 |
| UniProt Related Accession: | P28068 |
| Molecular Weight: | |
| NCBI Full Name: | HLA class II histocompatibility antigen, DM beta chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class II, DM beta |
| NCBI Official Symbol: | HLA-DMB |
| NCBI Official Synonym Symbols: | RING7; D6S221E |
| NCBI Protein Information: | HLA class II histocompatibility antigen, DM beta chain |
| UniProt Protein Name: | HLA class II histocompatibility antigen, DM beta chain |
| UniProt Synonym Protein Names: | MHC class II antigen DMB; Really interesting new gene 7 protein |
| Protein Family: | HLA class II histocompatibility antigen |
| UniProt Gene Name: | HLA-DMB |
| UniProt Entry Name: | DMB_HUMAN |
 | Western blot analysis of extracts of Raji cells, using HLA-DMB antibody (CAB8384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human stomach using HLA-DMB antibody (CAB8384) at dilution of 1:100 (40x lens). |