Anti-HLA-DMA Antibody (CAB6922)
- SKU:
- CAB6922
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-HLA-DMA Antibody |
| 抗体コード: | CAB6922 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-233 of human HLA-DMA (NP_006111.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | A-431, Jurkat, HeLa, Mouse lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-233 of human HLA-DMA (NP_006111.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VPEA PTPM WPDD LQNH TFLH TVYC QDGS PSVG LSEA YDED QLFF FDFS QNTR VPRL PEFA DWAQ EQGD APAI LFDK EFCE WMIQ QIGP KLDG KIPV SRGF PIAE VFTL KPLE FGKP NTLV CFVS NLFP PMLT VNWQ HHSV PVEG FGPT FVSA VDGL SFQA FSYL NFTP EPSD IFSC IVTH EIDR YTAI AYWV PRNA LPSD LLEN VLC |
| 遺伝子ID: | 3108 |
| Uniprot: | P28067 |
| セルラーロケーション: | Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein |
| 計算された分子量: | 29kDa |
| 観察された分子量: | 35kDa |
| 同義語: | HLA-DMA, D6S222E, DMA, HLADM, RING6, major histocompatibility complex, class II, DM alpha |
| バックグラウンド: | HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. |
| UniProt Protein Function: | Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. |
| NCBI Summary: | HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P28067 |
| NCBI GenInfo Identifier: | 133158 |
| NCBI Gene ID: | 3108 |
| NCBI Accession: | P28067.1 |
| UniProt Secondary Accession: | P28067,Q29639, Q29640, |
| UniProt Related Accession: | P28067 |
| Molecular Weight: | 28 |
| NCBI Full Name: | HLA class II histocompatibility antigen, DM alpha chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class II, DM alpha |
| NCBI Official Symbol: | HLA-DMA |
| NCBI Official Synonym Symbols: | DMA; HLADM; RING6; D6S222E |
| NCBI Protein Information: | HLA class II histocompatibility antigen, DM alpha chain |
| UniProt Protein Name: | HLA class II histocompatibility antigen, DM alpha chain |
| UniProt Synonym Protein Names: | MHC class II antigen DMA; Really interesting new gene 6 protein |
| UniProt Gene Name: | HLA-DMA |
 | Western blot analysis of extracts of various cell lines, using HLA-DMA antibody (CAB6922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |