Anti-HIRIP3 Antibody (CAB7014)
- SKU:
- CAB7014
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-HIRIP3 Antibody |
| 抗体コード: | CAB7014 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HL-60, HeLa, U-937, Jurkat, Mouse skeletal muscle, Mouse liver, Rat skeletal muscle |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GRRG EDHP AVMR LKRY IRAC GAHR NYKK LLGS CCSH KERL SILR AELE ALGM KGTP SLGK CRAL KEQR EEAA EVAS LDVA NIIS GSGR PRRR TAWN PLGE AAPP GELY RRTL DSDE ERPR PAPP DWSH MRGI ISSD GESN |
| 遺伝子ID: | 8479 |
| Uniprot: | Q9BW71 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 19kDa/27kDa/61kDa |
| 観察された分子量: | 62kDa |
| 同義語: | HIRIP3 |
| バックグラウンド: | The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | HIRIP3: May play a role in chromatin function and histone metabolism via its interaction with HIRA and histones. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: nucleoplasm; nucleolus; nucleus Molecular Function:protein binding Biological Process: chromatin assembly or disassembly |
| NCBI Summary: | The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Aug 2011] |
| UniProt Code: | Q9BW71 |
| NCBI GenInfo Identifier: | 116242511 |
| NCBI Gene ID: | 8479 |
| NCBI Accession: | Q9BW71.3 |
| UniProt Secondary Accession: | Q9BW71,O75707, O75708, H3BSR3, |
| UniProt Related Accession: | Q9BW71 |
| Molecular Weight: | 556 |
| NCBI Full Name: | HIRA-interacting protein 3 |
| NCBI Synonym Full Names: | HIRA interacting protein 3 |
| NCBI Official Symbol: | HIRIP3 |
| NCBI Protein Information: | HIRA-interacting protein 3 |
| UniProt Protein Name: | HIRA-interacting protein 3 |
| Protein Family: | HIRA-interacting protein |
| UniProt Gene Name: | HIRIP3 |
| UniProt Entry Name: | HIRP3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (CAB7014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using HIRIP3 antibody (CAB7014) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody (CAB7014) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using HIRIP3 antibody (CAB7014) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (CAB7014). |
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