Anti-GTF2E2 Antibody (CAB8732)
- SKU:
- CAB8732
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-GTF2E2 Antibody |
| 抗体コード: | CAB8732 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-291 of human GTF2E2 (NP_002086.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | A-375, NIH/3T3, HT-1080, Jurkat, 293T, Mouse brain, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-291 of human GTF2E2 (NP_002086.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDPS LLRE RELF KKRA LSTP VVEK RSAS SESS SSSS KKKK TKVE HGGS SGSK QNSD HSNG SFNL KALS GSSG YKFG VLAK IVNY MKTR HQRG DTHP LTLD EILD ETQH LDIG LKQK QWLM TEAL VNNP KIEV IDGK YAFK PKYN VRDK KALL RLLD QHDQ RGLG GILL EDIE EALP NSQK AVKA LGDQ ILFV NRPD KKKI LFFN DKSC QFSV DEEF QKLW RSVT VDSM DEEK IEEY LKRQ GISS MQES GPKK VAPI QRRK KPAS QKKR RFKT HNEH LAGV LKDY SDIT SSK |
| 遺伝子ID: | 2961 |
| Uniprot: | P29084 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 33kDa |
| 観察された分子量: | 37kDa |
| 同義語: | GTF2E2, FE, TF2E2, TFIIE-B, TTD6 |
| バックグラウンド: |
| UniProt Protein Function: | GTF2E2: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Belongs to the TFIIE beta subunit family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 8p12 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter |
| UniProt Code: | P29084 |
| NCBI GenInfo Identifier: | 135232 |
| NCBI Gene ID: | 2961 |
| NCBI Accession: | P29084.1 |
| UniProt Secondary Accession: | P29084,Q9H2B9, D3DSV2, |
| UniProt Related Accession: | P29084 |
| Molecular Weight: | 33,044 Da |
| NCBI Full Name: | Transcription initiation factor IIE subunit beta |
| NCBI Synonym Full Names: | general transcription factor IIE subunit 2 |
| NCBI Official Symbol: | GTF2E2 |
| NCBI Official Synonym Symbols: | FE; TTD6; TF2E2; TFIIE-B |
| NCBI Protein Information: | transcription initiation factor IIE subunit beta |
| UniProt Protein Name: | Transcription initiation factor IIE subunit beta |
| UniProt Synonym Protein Names: | General transcription factor IIE subunit 2 |
| Protein Family: | General transcription factor |
| UniProt Gene Name: | GTF2E2 |
| UniProt Entry Name: | T2EB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GTF2E2 antibody (CAB8732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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