Anti-GTF2A2 Antibody (CAB7680)
- SKU:
- CAB7680
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-GTF2A2 Antibody |
| 抗体コード: | CAB7680 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-109 of human GTF2A2 (NP_004483.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | NCI-H460 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-109 of human GTF2A2 (NP_004483.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAYQ LYRN TTLG NSLQ ESLD ELIQ SQQI TPQL ALQV LLQF DKAI NAAL AQRV RNRV NFRG SLNT YRFC DNVW TFVL NDVE FREV TELI KVDK VKIV ACDG KNTG SNTT E |
| 遺伝子ID: | 2958 |
| Uniprot: | P52657 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 12kDa |
| 観察された分子量: | 12kDa |
| 同義語: | GTF2A2, HsT18745, T18745, TF2A2, TFIIA, TFIIA-12, TFIIA-gamma, TFIIAS |
| バックグラウンド: |
| UniProt Protein Function: | GTF2A2: TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Belongs to the TFIIA subunit 2 family. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 15q22.2 Cellular Component: cell junction; nucleoplasm; transcription factor TFIIA complex Molecular Function:protein binding; protein heterodimerization activity; protein homodimerization activity; TATA-binding protein binding; transcription coactivator activity; transcription factor binding Biological Process: positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcriptional preinitiation complex assembly; viral reproduction |
| NCBI Summary: | Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | P52657 |
| NCBI GenInfo Identifier: | 49457380 |
| NCBI Gene ID: | 2958 |
| NCBI Accession: | CAG46989.1 |
| UniProt Related Accession: | P52657 |
| Molecular Weight: | |
| NCBI Full Name: | GTF2A2, partial |
| NCBI Synonym Full Names: | general transcription factor IIA subunit 2 |
| NCBI Official Symbol: | GTF2A2 |
| NCBI Official Synonym Symbols: | TF2A2; TFIIA; T18745; TFIIAS; HsT18745; TFIIA-12; TFIIA-gamma |
| NCBI Protein Information: | transcription initiation factor IIA subunit 2 |
| UniProt Protein Name: | Transcription initiation factor IIA subunit 2 |
| UniProt Synonym Protein Names: | General transcription factor IIA subunit 2; TFIIA p12 subunit; TFIIA-12; TFIIAS; Transcription initiation factor IIA gamma chain; TFIIA-gamma |
| UniProt Gene Name: | GTF2A2 |
| UniProt Entry Name: | T2AG_HUMAN |
 | Western blot analysis of extracts of NCI-H460 cells, using GTF2A2 antibody (CAB7680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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