Anti-GSR Antibody (CAB12070)
- SKU:
- CAB12070
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GSR Antibody |
| 抗体コード: | CAB12070 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 273-522 of human GSR (NP_000628.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | LO2, HeLa, A-549, Jurkat, Mouse brain, Mouse heart, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 273-522 of human GSR (NP_000628.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MIST NCTE ELEN AGVE VLKF SQVK EVKK TLSG LEVS MVTA VPGR LPVM TMIP DVDC LLWA IGRV PNTK DLSL NKLG IQTD DKGH IIVD EFQN TNVK GIYA VGDV CGKA LLTP VAIA AGRK LAHR LFEY KEDS KLDY NNIP TVVF SHPP IGTV GLTE DEAI HKYG IENV KTYS TSFT PMYH AVTK RKTK CVMK MVCA NKEE KVVG IHMQ GLGC DEML QGFA VAVK MGAT KADF DNTV AIHP TSSE ELVT LR |
| 遺伝子ID: | 2936 |
| Uniprot: | P00390 |
| セルラーロケーション: | Cytoplasm, Mitochondrion |
| 計算された分子量: | 47kDa/50kDa/51kDa/53kDa/56kDa |
| 観察された分子量: | 55kDa |
| 同義語: | GSR, HEL-75, HEL-S-122m |
| バックグラウンド: | This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. |
| UniProt Protein Function: | GSR: a class-I pyridine nucleotide-disulfide oxidoreductase. Maintains high levels of reduced glutathione in the cytosol. Alternative initiation results in a mitochondrial and a cytoplasmic isoform. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - glutathione; Oxidoreductase; EC 1.8.1.7 Chromosomal Location of Human Ortholog: 8p21.1 Cellular Component: cytosol; mitochondrial matrix Molecular Function:electron carrier activity; glutathione-disulfide reductase activity Biological Process: nucleobase, nucleoside and nucleotide interconversion; response to reactive oxygen species |
| NCBI Summary: | This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010] |
| UniProt Code: | P00390 |
| NCBI GenInfo Identifier: | 14916998 |
| NCBI Gene ID: | 2936 |
| NCBI Accession: | P00390.2 |
| UniProt Secondary Accession: | P00390,Q7Z5C9, Q9NP63, C8KIL8, C8KIL9, C8KIM0, D3DSV3 |
| UniProt Related Accession: | P00390 |
| Molecular Weight: | 47,267 Da |
| NCBI Full Name: | Glutathione reductase, mitochondrial |
| NCBI Synonym Full Names: | glutathione-disulfide reductase |
| NCBI Official Symbol: | GSR |
| NCBI Official Synonym Symbols: | HEL-75; HEL-S-122m |
| NCBI Protein Information: | glutathione reductase, mitochondrial |
| UniProt Protein Name: | Glutathione reductase, mitochondrial |
| Protein Family: | Glutathione reductase |
| UniProt Gene Name: | GSR |
| UniProt Entry Name: | GSHR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GSR antibody (CAB12070) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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