Anti-GRIN2A Antibody (CAB0924)
- SKU:
- CAB0924
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| 抗体名: | Anti-GRIN2A Antibody |
| 抗体コード: | CAB0924 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1130-1400 of human GRIN2A (NP_000824.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1130-1400 of human GRIN2A (NP_000824.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LDPP QFVE NVTL PENV DFPD PYQD PSEN FRKG DSTL PMNR NPLH NEEG LSNN DQYK LYSK HFTL KDKG SPHS ETSE RYRQ NSTH CRSC LSNM PTYS GHFT MRSP FKCD ACLR MGNL YDID EDQM LQET GNPA TGEQ VYQQ DWAQ NNAL QLQK NKLR ISRQ HSYD NIVD KPRE LDLS RPSR SISL KDRE RLLE GNFY GSLF SVPS SKLS GKKS SLFP QGLE DSKR SKSL LPDH TSDN PFLH SHRD DQRL VIGR CPSD PYKH SLPS QAVN DSY |
| 遺伝子ID: | 2903 |
| Uniprot: | Q12879 |
| セルラーロケーション: | Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse |
| 計算された分子量: | 144kDa/165kDa |
| 観察された分子量: | 200kDa |
| 同義語: | EPND, FESD, GluN2A, LKS, NMDAR2A, NR2A, GRIN2A, NMDA 2A |
| バックグラウンド: | This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated Chromosomal Location of Human Ortholog: 16p13.2 Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; cell surface; integral to plasma membrane; endoplasmic reticulum; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex Molecular Function:protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity Biological Process: startle response; positive regulation of apoptosis; regulation of synaptic plasticity; sensory perception of pain; dopamine metabolic process; synaptic transmission; protein localization; learning and/or memory; transport; response to wounding; visual learning; serotonin metabolic process; response to drug; synaptic transmission, glutamatergic; glutamate signaling pathway; response to amphetamine; sleep; memory; response to ethanol; neurogenesis; ionotropic glutamate receptor signaling pathway; directional locomotion; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; negative regulation of protein catabolic process Disease: Epilepsy, Focal, With Speech Disorder And With Or Without Mental Retardation |
| NCBI Summary: | This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| UniProt Code: | Q12879 |
| NCBI GenInfo Identifier: | 14285603 |
| NCBI Gene ID: | 2903 |
| NCBI Accession: | Q12879.1 |
| UniProt Secondary Accession: | Q12879,O00669, Q17RZ6, |
| UniProt Related Accession: | Q12879 |
| Molecular Weight: | 144,431 Da |
| NCBI Full Name: | Glutamate receptor ionotropic, NMDA 2A |
| NCBI Synonym Full Names: | glutamate receptor, ionotropic, N-methyl D-aspartate 2A |
| NCBI Official Symbol: | GRIN2A |
| NCBI Official Synonym Symbols: | LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A |
| NCBI Protein Information: | glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; N-methyl-D-aspartate receptor channel, subunit epsilon-1 |
| UniProt Protein Name: | Glutamate receptor ionotropic, NMDA 2A |
| UniProt Synonym Protein Names: | Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A; hNR2A |
| Protein Family: | Glutamate receptor ionotropic |
| UniProt Gene Name: | GRIN2A |
| UniProt Entry Name: | NMDE1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GRIN2A antibody (CAB0924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GRIN2A antibody (CAB0924) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of H9C2 cells using GRIN2A Rabbit pAb (CAB0924) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using GRIN2A Rabbit pAb (CAB0924) at dilution of 1:100. Blue: DAPI for nuclear staining. |