Description
| 商品名: | Anti-GPC3 Antibody |
| Product SKU | RACO0584 |
| サイズ: | 50 µL |
| 申し込み: | ELISA, WB, IF |
| 推奨される希釈: | Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human GPC3 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. |
| シノニム: | DGSX antibody, Glypican proteoglycan 3 antibody, Glypican-3, Gpc3 antibody, GTR2 2 antibody; GTR2-2 antibody, Heparan sulphate proteoglycan antibody, Intestinal protein OCI 5 antibody, MXR7 antibody, OCI5 antibody, SDYS antibody, Secreted glypican-3 antibody, SGB antibody, SGBS antibody, SGBS1 antibody |
 | Western Blot Positive WB detected in: 293 whole cell lysate, K562 whole cell lysate All lanes: GPC3 antibody at 0.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66, 60, 69 KDa Observed band size: 66 KDa |
 | Immunofluorescence staining of HepG2 cells with RACO0584 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| UniProt Protein Function: | GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: Xq26.1 Cellular Component: extracellular space; proteinaceous extracellular matrix; lysosomal lumen; anchored to plasma membrane; integral to plasma membrane; Golgi lumen; plasma membrane Molecular Function:heparan sulfate proteoglycan binding; protein binding Biological Process: phototransduction, visible light; anatomical structure morphogenesis; glycosaminoglycan metabolic process; negative regulation of peptidase activity; pathogenesis; positive regulation of endocytosis; osteoclast differentiation; embryonic hindlimb morphogenesis; body morphogenesis; bone mineralization; chondroitin sulfate metabolic process; positive regulation of glucose import; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; negative regulation of growth; anterior/posterior axis specification; negative regulation of epithelial cell proliferation; lung development Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1 |
| NCBI Summary: | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| UniProt Code: | P51654 |
| NCBI GenInfo Identifier: | 2599578 |
| NCBI Gene ID: | 2719 |
| NCBI Accession: | |
| UniProt Related Accession: | P51654,Q2L880,Q2L882,Q53H15,Q8IYG2 |
| Molecular Weight: | 65,563 Da |
| NCBI Full Name: | glypican 3 |
| NCBI Synonym Full Names: | glypican 3 |
| NCBI Official Symbol: | GPC3 |
| NCBI Official Synonym Symbols: | SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; GPC3 |
| NCBI Protein Information: | glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan |
| UniProt Protein Name: | Glypican-3 |
| UniProt Synonym Protein Names: | GTR2-2; Intestinal protein OCI-5; MXR7 |
| Protein Family: | Glypican |
| UniProt Gene Name: | GPC3 |
| UniProt Entry Name: | GPC3_HUMAN |