Anti-GOLPH3 Antibody (CAB13121)
- SKU:
- CAB13121
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GOLPH3 Antibody |
| 抗体コード: | CAB13121 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human GOLPH3 (NP_071413.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 22Rv1, LO2, HeLa, Mouse testis, Rat lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human GOLPH3 (NP_071413.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MTSL TQRS SGLV QRRT EASR NAAD KERA AGGG AGSS EDDA QSRR DEQD DDDK GDSK ETRL TLME EVLL LGLK DREG YTSF WNDC ISSG LRGC MLIE LALR GRLQ LEAC GMRR KSLL TRKV ICKS DAPT GDVL LDEA LKHV KETQ PPET VQNW IELL SGET WNPL KLHY QLRN VRER LAKN LVEK GVLT TEKQ NFLL FDMT THPL TNNN IKQR LIKK VQEA VLDK WVND PHRM DRRL LALI YLAH ASDV LENA FAPL LDEQ YDLA TKRV RQLL DLDP EVEC LKAN TNEV LWAV VAAF TK |
| 遺伝子ID: | 64083 |
| Uniprot: | Q9H4A6 |
| セルラーロケーション: | Cell membrane, Cytoplasmic side, Endosome, Golgi apparatus, Golgi stack membrane, Mitochondrion intermembrane space, Peripheral membrane protein, trans-Golgi network membrane |
| 計算された分子量: | 33kDa |
| 観察された分子量: | 37kDa |
| 同義語: | GOLPH3, GOPP1, GPP34, MIDAS, Vps74 |
| バックグラウンド: | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. |
| UniProt Protein Function: | GOLPH3: Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. Belongs to the GOLPH3/VPS74 family. |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 5p13.3 Cellular Component: cytosol; Golgi apparatus; Golgi cisterna; intracellular membrane-bound organelle; mitochondrion; nucleoplasm; trans-Golgi network Molecular Function:enzyme binding; protein binding Biological Process: cell migration; cell proliferation; gene expression; glycoprotein biosynthetic process; Golgi organization and biogenesis; Golgi to plasma membrane protein transport; Golgi vesicle budding; lamellipodium biogenesis; leukocyte tethering or rolling; negative regulation of apoptosis; positive regulation of protein secretion; positive regulation of TOR signaling pathway; protein retention in Golgi; protein secretion |
| NCBI Summary: | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H4A6 |
| NCBI GenInfo Identifier: | 50400651 |
| NCBI Gene ID: | 64083 |
| NCBI Accession: | Q9H4A6.1 |
| UniProt Secondary Accession: | Q9H4A6,Q9UIW5, |
| UniProt Related Accession: | Q9H4A6 |
| Molecular Weight: | 33,811 Da |
| NCBI Full Name: | Golgi phosphoprotein 3 |
| NCBI Synonym Full Names: | golgi phosphoprotein 3 |
| NCBI Official Symbol: | GOLPH3 |
| NCBI Official Synonym Symbols: | GOPP1; GPP34; MIDAS; Vps74 |
| NCBI Protein Information: | Golgi phosphoprotein 3 |
| UniProt Protein Name: | Golgi phosphoprotein 3 |
| UniProt Synonym Protein Names: | Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS |
| Protein Family: | Golgi phosphoprotein |
| UniProt Gene Name: | GOLPH3 |
| UniProt Entry Name: | GOLP3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GOLPH3 antibody (CAB13121) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of L929 cells using GOLPH3 antibody (CAB13121) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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