Anti-GOLGA4 Antibody (CAB10216)
- SKU:
- CAB10216
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GOLGA4 Antibody |
| 抗体コード: | CAB10216 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GOLGA4 (NP_002069.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, HeLa, SW480, A-549, MCF7, Mouse heart, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GOLGA4 (NP_002069.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MFKK LKQK ISEE QQQL QQAL APAQ ASSN SSTP TRMR SRTS SFTE QLDE GTPN RESG DTQS FAQK LQLR VPSV ESLF RSPI KESL FRSS SKES LVRT SSRE SLNR LDLD SSTA SFDP PSDM DSEA EDLV GNSD SLNK EQLI QRLR RMER SL |
| 遺伝子ID: | 2803 |
| Uniprot: | Q13439 |
| セルラーロケーション: | Cytoplasm, Golgi apparatus, Golgi apparatus membrane, Peripheral membrane protein |
| 計算された分子量: | 260kDa/261kDa/262kDa |
| 観察された分子量: | 280kDa |
| 同義語: | GOLGA4, CRPF46, GCP2, GOLG, MU-RMS-40.18, p230, golgin A4 |
| バックグラウンド: | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein has been postulated to play a role in Rab6-regulated membrane-tethering events in the Golgi apparatus. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. |
| UniProt Protein Function: | GOLGA4: May play a role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with MACF1. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 3p22.2 Cellular Component: cytoplasm; cytosol; Golgi apparatus; intracellular membrane-bound organelle; trans-Golgi network Molecular Function:GTPase binding; protein binding Biological Process: Golgi to plasma membrane protein transport; positive regulation of axon extension; vesicle-mediated transport |
| NCBI Summary: | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein has been postulated to play a role in Rab6-regulated membrane-tethering events in the Golgi apparatus. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010] |
| UniProt Code: | Q13439 |
| NCBI GenInfo Identifier: | 12643718 |
| NCBI Gene ID: | 2803 |
| NCBI Accession: | Q13439.1 |
| UniProt Secondary Accession: | Q13439,Q13270, Q13654, Q14436, Q59EW8, F8W8Q7, |
| UniProt Related Accession: | Q13439 |
| Molecular Weight: | 261kDa |
| NCBI Full Name: | Golgin subfamily A member 4 |
| NCBI Synonym Full Names: | golgin A4 |
| NCBI Official Symbol: | GOLGA4 |
| NCBI Official Synonym Symbols: | GCP2; GOLG; p230; CRPF46; MU-RMS-40.18 |
| NCBI Protein Information: | golgin subfamily A member 4 |
| UniProt Protein Name: | Golgin subfamily A member 4 |
| UniProt Synonym Protein Names: | 256 kDa golgin; Golgin-245; Protein 72.1; Trans-Golgi p230 |
| Protein Family: | Golgin subfamily |
| UniProt Gene Name: | GOLGA4 |
 | Western blot analysis of extracts of various cell lines, using GOLGA4 antibody (CAB10216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of C6 cells using GOLGA4 Rabbit pAb (CAB10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using GOLGA4 Rabbit pAb (CAB10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using GOLGA4 Rabbit pAb (CAB10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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