Anti-GNAI3 Antibody (CAB1922)
- SKU:
- CAB1922
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-GNAI3 Antibody |
| 抗体コード: | CAB1922 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAI3 (NP_006487.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:1000 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | A-431, Rat kidney |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAI3 (NP_006487.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGCT LSAE DKAA VERS KMID RNLR EDGE KAAK EVKL LLLG AGES GKST IVKQ MKII HEDG YSED ECKQ YKVV VYSN TIQS IIAI IRAM GRLK IDFG EAAR |
| 遺伝子ID: | 2773 |
| Uniprot: | P08754 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 40kDa |
| 観察された分子量: | 41-45kDa |
| 同義語: | GNAI3, 87U6, ARCND1 |
| バックグラウンド: | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. |
| UniProt Protein Function: | G-alpha i3: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor- regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. Defects in GNAI3 are the cause of auriculocondylar syndrome 1 (ARCND1). ARCND1 is an autosomal dominant craniofacial malformation syndrome characterized by variable mandibular anomalies, including mild to severe micrognathia, temporomandibular joint ankylosis, cleft palate, and a characteristic ear malformation that consists of separation of the lobule from the external ear, giving the appearance of a question mark (question-mark ear). Other frequently described features include prominent cheeks, cupped and posteriorly rotated ears, preauricular tags, and microstomia. Belongs to the G-alpha family. G(i/o/t/z) subfamily. |
| UniProt Protein Details: | Protein type:G protein; G protein, heterotrimeric alpha G((i/o/t/z)); G protein, heterotrimeric Chromosomal Location of Human Ortholog: 1p13 Cellular Component: centrosome; cytoplasm; heterotrimeric G-protein complex; lysosomal membrane; membrane; midbody; plasma membrane Molecular Function:G-protein beta/gamma-subunit binding; G-protein-coupled receptor binding; GDP binding; GTP binding; GTPase activity; protein binding; signal transducer activity Biological Process: cell division; dopamine receptor signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; GTP metabolic process; negative regulation of adenylate cyclase activity; protein folding Disease: Auriculocondylar Syndrome 1 |
| NCBI Summary: | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012] |
| UniProt Code: | P08754 |
| NCBI GenInfo Identifier: | 120996 |
| NCBI Gene ID: | 2773 |
| NCBI Accession: | P08754.3 |
| UniProt Secondary Accession: | P08754,P17539, Q5TZX1, |
| UniProt Related Accession: | P08754 |
| Molecular Weight: | 40,532 Da |
| NCBI Full Name: | Guanine nucleotide-binding protein G(k) subunit alpha |
| NCBI Synonym Full Names: | G protein subunit alpha i3 |
| NCBI Official Symbol: | GNAI3 |
| NCBI Official Synonym Symbols: | 87U6; ARCND1 |
| NCBI Protein Information: | guanine nucleotide-binding protein G(k) subunit alpha |
| UniProt Protein Name: | Guanine nucleotide-binding protein G(k) subunit alpha |
| UniProt Synonym Protein Names: | G(i) alpha-3 |
| UniProt Gene Name: | GNAI3 |
| UniProt Entry Name: | GNAI3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GNAI3 antibody (CAB1922) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using GNAI3 antibody (CAB1922) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using GNAI3 antibody (CAB1922) at dilution of 1:100 (40x lens). |
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