Anti-GNAI2 Antibody (CAB14547)
- SKU:
- CAB14547
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| 抗体名: | Anti-GNAI2 Antibody |
| 抗体コード: | CAB14547 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-355 of human GNAI2 (NP_002061.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, Mouse brain, Mouse kidney, Rat lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-355 of human GNAI2 (NP_002061.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGCT VSAE DKAA AERS KMID KNLR EDGE KAAR EVKL LLLG AGES GKST IVKQ MKII HEDG YSEE ECRQ YRAV VYSN TIQS IMAI VKAM GNLQ IDFA DPSR ADDA RQLF ALSC TAEE QGVL PDDL SGVI RRLW ADHG VQAC FGRS REYQ LNDS AAYY LNDL ERIA QSDY IPTQ QDVL RTRV KTTG IVET HFTF KDLH FKMF DVGG QRSE RKKW IHCF EGVT AIIF CVAL SAYD LVLA EDEE MNRM HESM KLFD SICN NKWF TDTS IILF LNKK DLFE EKIT HSPL TICF PEYT GANK YDEA ASYI QSKF EDLN KRKD TKEI YTHF TCAT DTKN VQFV FDAV TDVI IKNN LKDC GLF |
| 遺伝子ID: | 2771 |
| Uniprot: | P04899 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 34kDa/36kDa/38kDa/40kDa/41kDa |
| 観察された分子量: | 40kDa |
| 同義語: | GNAI2, GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1 |
| バックグラウンド: | The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | G-alpha i2: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. Belongs to the G-alpha family. G(i/o/t/z) subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:G protein; G protein, heterotrimeric; G protein, heterotrimeric alpha G((i/o/t/z)) Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: nucleoplasm; centrosome; membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; midbody; cytosol; vesicle; lipid raft Molecular Function:GTPase activity; signal transducer activity; protein binding; G-protein-coupled receptor binding; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding Biological Process: platelet activation; acetylcholine receptor signaling, muscarinic pathway; activation of MAPKK activity; metabolic process; negative regulation of adenylate cyclase activity; regulation of calcium ion transport; cell cycle; signal transduction; negative regulation of synaptic transmission; G-protein coupled receptor protein signaling pathway; synaptic transmission; cell proliferation; cell division; positive regulation of cell proliferation; G-protein signaling, adenylate cyclase inhibiting pathway; blood coagulation; adenosine receptor signaling pathway; gamma-aminobutyric acid signaling pathway; response to nutrient Disease: Ventricular Tachycardia, Familial |
| NCBI Summary: | The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
| UniProt Code: | P04899 |
| NCBI GenInfo Identifier: | 121023 |
| NCBI Gene ID: | 2771 |
| NCBI Accession: | P04899.3 |
| UniProt Secondary Accession: | P04899,Q6B6N3, Q8IZ71, B3KTZ0, B4DYA0, B4E2X5, |
| UniProt Related Accession: | P04899 |
| Molecular Weight: | 355 |
| NCBI Full Name: | Guanine nucleotide-binding protein G(i) subunit alpha-2 |
| NCBI Synonym Full Names: | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 |
| NCBI Official Symbol: | GNAI2 |
| NCBI Official Synonym Symbols: | GIP; GNAI2B; H_LUCA15.1; H_LUCA16.1 |
| NCBI Protein Information: | guanine nucleotide-binding protein G(i) subunit alpha-2; guanine nucleotide-binding protein G(i) subunit alpha-2; adenylate cyclase-inhibiting G alpha protein; GTP-binding regulatory protein Gi alpha-2 chain; guanine nucleotide-binding protein G(i), alpha |
| UniProt Protein Name: | Guanine nucleotide-binding protein G(i) subunit alpha-2 |
| UniProt Synonym Protein Names: | Adenylate cyclase-inhibiting G alpha protein |
| UniProt Gene Name: | GNAI2 |
| UniProt Entry Name: | GNAI2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GNAI2 antibody (CAB14547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using GNAI2 Polyclonal Antibody (CAB14547) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using GNAI2 Polyclonal Antibody (CAB14547) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using GNAI2 Polyclonal Antibody (CAB14547) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |