Anti-GNAI1 Antibody (CAB8844)
- SKU:
- CAB8844
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-GNAI1 Antibody |
| 抗体コード: | CAB8844 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI1 (NP_002060.4). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, Mouse brain, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI1 (NP_002060.4). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGCT LSAE DKAA VERS KMID RNLR EDGE KAAR EVKL LLLG AGES GKST IVKQ MKII HEAG YSEE ECKQ YKAV VYSN TIQS IIAI IRAM GRLK IDFG DSAR ADDA RQLF VLAG AAEE GFMT AELA GVIK RLWK DSGV QACF NRSR EYQL NDSA AYYL NDLD RIAQ PNYI PTQQ DVLR TRVK TTGI VETH FTFK DLHF KMFD VGGQ RSER KKWI HCFE GVTA IIFC VALS DYDL VLAE DEEM NRMH ESMK LFDS ICNN KWFT DTSI ILFL NKKD LFEE KIKK SPLT ICYP EYAG SNTY EEAA AYIQ CQFE DLNK RKDT KEIY THFT CATD TKNV QFVF DAVT DVII KNNL KDCG LF |
| 遺伝子ID: | 2770 |
| Uniprot: | P63096 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Lipid-anchor, Membrane, Nucleus, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 34kDa/40kDa |
| 観察された分子量: | 40kDa |
| 同義語: | GNAI1, Gi |
| バックグラウンド: | Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | G-alpha i1: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. Interacts with GPSM1. Interacts with RGS12. Interacts (via active GTP- or inactive GDP-bound forms) with RGS14 (via RGS and GoLoco domains); the interaction occurs in the centrosomes. Interacts (GDP-bound form) with RIC8A (via C-terminus). Interacts with DRD2. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily. |
| UniProt Protein Details: | Protein type:G protein, heterotrimeric; G protein; G protein, heterotrimeric alpha G((i/o/t/z)) Chromosomal Location of Human Ortholog: 7q21 Cellular Component: centrosome; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; midbody; nucleus Molecular Function:GTPase activity; protein binding; signal transducer activity; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; metabotropic serotonin receptor binding Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; G-protein coupled receptor protein signaling pathway; response to peptide hormone stimulus; platelet activation; metabolic process; cell division; G-protein signaling, adenylate cyclase inhibiting pathway; blood coagulation; cell cycle |
| NCBI Summary: | Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P63096 |
| NCBI GenInfo Identifier: | 52000964 |
| NCBI Gene ID: | 2770 |
| NCBI Accession: | P63096.2 |
| UniProt Secondary Accession: | P63096,P04898, P11015, P31871, Q5U074, Q8TAN5, Q9UGA4 A8KA88, B4E2V1, C9J3A4, |
| UniProt Related Accession: | P63096 |
| Molecular Weight: | 34,775 Da |
| NCBI Full Name: | Guanine nucleotide-binding protein G(i) subunit alpha-1 |
| NCBI Synonym Full Names: | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 |
| NCBI Official Symbol: | GNAI1 |
| NCBI Official Synonym Symbols: | Gi |
| NCBI Protein Information: | guanine nucleotide-binding protein G(i) subunit alpha-1; guanine nucleotide-binding protein G(i) subunit alpha-1; Gi1 protein alpha subunit; adenylate cyclase-inhibiting G alpha protein |
| UniProt Protein Name: | Guanine nucleotide-binding protein G(i) subunit alpha-1 |
| UniProt Synonym Protein Names: | Adenylate cyclase-inhibiting G alpha protein |
| UniProt Gene Name: | GNAI1 |
| UniProt Entry Name: | GNAI1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GNAI1 antibody (CAB8844) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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