Anti-GLS2 Antibody (CAB16029)
- SKU:
- CAB16029
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GLS2 Antibody |
| 抗体コード: | CAB16029 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide of human GLS2 |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:1000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: |
| 免疫原: | A synthetic peptide of human GLS2 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 27165 |
| Uniprot: | Q9UI32 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 23kDa/66kDa |
| 観察された分子量: | 66kDa |
| 同義語: | GLS2, GA, GLS, LGA, hLGA |
| バックグラウンド: | The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. |
| UniProt Protein Function: | GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; EC 3.5.1.2; Energy Metabolism - nitrogen; Hydrolase; Mitochondrial; Other Amino Acids Metabolism - D-Glutamine and D-glutamate Chromosomal Location of Human Ortholog: 12q13.3 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:glutaminase activity; protein binding Biological Process: amino acid biosynthetic process; amino acid metabolic process; glutamate biosynthetic process; glutamate secretion; glutamine catabolic process; regulation of apoptosis |
| NCBI Summary: | The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9UI32 |
| NCBI GenInfo Identifier: | 145559477 |
| NCBI Gene ID: | 27165 |
| NCBI Accession: | Q9UI32.2 |
| UniProt Secondary Accession: | Q9UI32,Q8IX91, Q9NYY2, Q9UI31, B7Z8Q9, |
| UniProt Related Accession: | Q9UI32 |
| Molecular Weight: | 66kDa |
| NCBI Full Name: | Glutaminase liver isoform, mitochondrial |
| NCBI Synonym Full Names: | glutaminase 2 |
| NCBI Official Symbol: | GLS2 |
| NCBI Official Synonym Symbols: | GA; GLS; LGA; hLGA |
| NCBI Protein Information: | glutaminase liver isoform, mitochondrial |
| UniProt Protein Name: | Glutaminase liver isoform, mitochondrial |
| UniProt Synonym Protein Names: | L-glutaminase; L-glutamine amidohydrolase |
| Protein Family: | Glucosidase |
| UniProt Gene Name: | GLS2 |
 | Western blot analysis of extracts of various cell lines, using GLS2 antibody (CAB16029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
View AllClose