Anti-GLRX2 Antibody (CAB6603)
- SKU:
- CAB6603
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GLRX2 Antibody |
| 抗体コード: | CAB6603 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human GLRX2 (NP_001230328.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human GLRX2 (NP_001230328.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MESN TSSS LENL ATAP VNQI QETI SDNC VVIF SKTS CSYC TMAK KLFH DMNV NYKV VELD LLEY GNQF QDAL YKMT GERT VPRI FVNG TFIG GATD THRL HKEG KLLP LVHQ CYLK KSKR KEFQ |
| 遺伝子ID: | 51022 |
| Uniprot: | Q9NS18 |
| セルラーロケーション: | Mitochondrion, Nucleus |
| 計算された分子量: | 18kDa |
| 観察された分子量: | 18kDa |
| 同義語: | GLRX2, CGI-133, GRX2 |
| バックグラウンド: | The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression. |
| UniProt Protein Function: | GLRX2: Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release. Belongs to the glutaredoxin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Mitochondrial Chromosomal Location of Human Ortholog: 1q31.2 Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; nucleus Molecular Function:2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; protein disulfide oxidoreductase activity; protein disulfide isomerase activity; arsenate reductase (glutaredoxin) activity; glutathione disulfide oxidoreductase activity Biological Process: response to organic substance; response to temperature stimulus; DNA protection; glutathione metabolic process; response to hydrogen peroxide; regulation of transcription, DNA-dependent; protein folding; apoptosis; cell redox homeostasis; regulation of signal transduction; response to redox state; cell differentiation |
| NCBI Summary: | The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q9NS18 |
| NCBI GenInfo Identifier: | 73919686 |
| NCBI Gene ID: | 51022 |
| NCBI Accession: | Q9NS18.1 |
| UniProt Secondary Accession: | Q9NS18,Q3LR69, Q7L1N7, Q96JC0, Q9Y3D4, |
| UniProt Related Accession: | Q9NS18 |
| Molecular Weight: | 18,726 Da |
| NCBI Full Name: | Glutaredoxin-2, mitochondrial |
| NCBI Synonym Full Names: | glutaredoxin 2 |
| NCBI Official Symbol: | GLRX2 |
| NCBI Official Synonym Symbols: | GRX2; CGI-133 |
| NCBI Protein Information: | glutaredoxin 2; bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2) |
| UniProt Protein Name: | Glutaredoxin-2, mitochondrial |
| UniProt Gene Name: | GLRX2 |
| UniProt Entry Name: | GLRX2_HUMAN |
 | Western blot analysis of extracts of MCF-7 cells, using GLRX2 Antibody (CAB6603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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