Anti-GLIS3 Antibody (CAB12166)
- SKU:
- CAB12166
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-GLIS3 Antibody |
| 抗体コード: | CAB12166 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 606-775 of human GLIS3 (NP_689842.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | U-87MG, BxPC-3, HepG2, A-549, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 606-775 of human GLIS3 (NP_689842.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DAGA ERFA PSAP SPHH ISPR RVPA PSSI LQRT QPPY TQQP SGSH LKSY QPET NSSF QPNG IHVH GFYG QLQK FCPP HYPD SQRI VPPV SSCS VVPS FEDC LVPT SMGQ ASFD VFHR AFST HSGI TVYD LPSS SSSL FGES LRSG AEDA TFLQ ISTV DRCP SQLS SVYT EG |
| 遺伝子ID: | 169792 |
| Uniprot: | Q8NEA6 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 83kDa/99kDa |
| 観察された分子量: | 72kDa |
| 同義語: | GLIS3, NDH, ZNF515 |
| バックグラウンド: | This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. |
| UniProt Protein Function: | GLIS3: Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'. Defects in GLIS3 are a cause of diabetes mellitus neonatal with congenital hypothyroidism (NDH). NDH is a neonatal diabetes syndrome associated with congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; Transcription factor Chromosomal Location of Human Ortholog: 9p24.2 Cellular Component: nucleus Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter Disease: Diabetes Mellitus, Neonatal, With Congenital Hypothyroidism |
| NCBI Summary: | This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8NEA6 |
| NCBI GenInfo Identifier: | 322510143 |
| NCBI Gene ID: | 169792 |
| NCBI Accession: | Q8NEA6.5 |
| UniProt Secondary Accession: | Q8NEA6,Q1PHK5, B1AL19, |
| UniProt Related Accession: | Q8NEA6 |
| Molecular Weight: | 84kDa |
| NCBI Full Name: | Zinc finger protein GLIS3 |
| NCBI Synonym Full Names: | GLIS family zinc finger 3 |
| NCBI Official Symbol: | GLIS3 |
| NCBI Official Synonym Symbols: | NDH; ZNF515 |
| NCBI Protein Information: | zinc finger protein GLIS3 |
| UniProt Protein Name: | Zinc finger protein GLIS3 |
| UniProt Synonym Protein Names: | GLI-similar 3; Zinc finger protein 515 |
| Protein Family: | Zinc finger protein |
| UniProt Gene Name: | GLIS3 |
 | Western blot analysis of extracts of various cell lines, using GLIS3 antibody (CAB12166) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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