Anti-GFRA1 Antibody (CAB5373)
- SKU:
- CAB5373
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GFRA1 Antibody |
| 抗体コード: | CAB5373 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human GFRA1 (NP_665736.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human GFRA1 (NP_665736.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DRLD CVKA SDQC LKEQ SCST KYRT LRQC VAGK ETNF SLAS GLEA KDEC RSAM EALK QKSL YNCR CKRG MKKE KNCL RIYW SMYQ SLQG NDLL EDSP YEPV NSRL SDIF RVVP FISV EHIP KGNN CLDA AKAC NLDD ICKK YRSA YITP CTTS VSND VCNR RKCH KALR QFFD KVPA KHSY GMLF CSCR DIAC TERR RQTI VPVC SYEE REKP NCLN LQDS CKTN YICR SRLA DFFT NCQP ESRS VSSC LKEN YADC |
| 遺伝子ID: | 2674 |
| Uniprot: | P56159 |
| セルラーロケーション: | Cell membrane, GPI-anchor, Lipid-anchor |
| 計算された分子量: | 50kDa/51kDa |
| 観察された分子量: | 60kDa |
| 同義語: | GFRA1, GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1 |
| バックグラウンド: | This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. |
| UniProt Protein Function: | GFRA1: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor; Receptor, misc. Chromosomal Location of Human Ortholog: 10q26.11 Cellular Component: extrinsic to membrane; plasma membrane Molecular Function:glial cell line-derived neurotrophic factor receptor activity; receptor binding Biological Process: axon guidance; cell surface receptor linked signal transduction |
| NCBI Summary: | Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Feb 2009] |
| UniProt Code: | P56159 |
| NCBI GenInfo Identifier: | 20141405 |
| NCBI Gene ID: | 2674 |
| NCBI Accession: | P56159.2 |
| UniProt Secondary Accession: | P56159,O15507, O43912, A8KA21, |
| UniProt Related Accession: | P56159 |
| Molecular Weight: | |
| NCBI Full Name: | GDNF family receptor alpha-1 |
| NCBI Synonym Full Names: | GDNF family receptor alpha 1 |
| NCBI Official Symbol: | GFRA1 |
| NCBI Official Synonym Symbols: | GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1 |
| NCBI Protein Information: | GDNF family receptor alpha-1; RET ligand 1; GDNFR-alpha-1; GDNF receptor alpha-1; GPI-linked anchor protein; PI-linked cell-surface accessory protein; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; Glial cell line-derived neurotrophic factor receptor alpha |
| UniProt Protein Name: | GDNF family receptor alpha-1 |
| UniProt Synonym Protein Names: | RET ligand 1; TGF-beta-related neurotrophic factor receptor 1 |
| Protein Family: | GDNF family receptor |
| UniProt Gene Name: | GFRA1 |
| UniProt Entry Name: | GFRA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GFRA1 antibody (CAB5373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human thyroid cancer using GFRA1 Rabbit pAb (CAB5373) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using GFRA1 Rabbit pAb (CAB5373) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat testis using GFRA1 Rabbit pAb (CAB5373) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using GFRA1 Rabbit pAb (CAB5373) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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