Anti-GFAP Antibody (CAB14673)
- SKU:
- CAB14673
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GFAP Antibody |
| 抗体コード: | CAB14673 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | mouse brain, rat brain |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RGTN ESLE RQMR EQEE RHVR EAAS YQEA LARL EEEG QSLK DEMA RHLQ EYQD LLNV KLAL DIEI ATYR KLLE GEEN RITI PVQT FSNL QIRE TSLD TKSV SEGH LKRN IVVK TVEM RDGE VIKE SKQE HKDV M |
| 遺伝子ID: | 2670 |
| Uniprot: | P14136 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 49kDa/50kDa |
| 観察された分子量: | 50kDa |
| 同義語: | ALXDRD, GFAP |
| バックグラウンド: | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 17q21 Cellular Component: membrane; cytoplasm; intermediate filament; cytosol Molecular Function:integrin binding; structural constituent of cytoskeleton; kinase binding Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; intermediate filament organization; neurite regeneration; astrocyte development Disease: Alexander Disease |
| NCBI Summary: | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P14136 |
| NCBI GenInfo Identifier: | 121135 |
| NCBI Gene ID: | 2670 |
| NCBI Accession: | P14136.1 |
| UniProt Secondary Accession: | P14136,Q53H98, Q5D055, Q6ZQS3, Q7Z5J6, Q7Z5J7, Q96KS4 Q96P18, Q9UFD0, B2RD44, D3DX59, E9PAX3, |
| UniProt Related Accession: | P14136 |
| Molecular Weight: | 432 |
| NCBI Full Name: | Glial fibrillary acidic protein |
| NCBI Synonym Full Names: | glial fibrillary acidic protein |
| NCBI Official Symbol: | GFAP |
| NCBI Protein Information: | glial fibrillary acidic protein |
| UniProt Protein Name: | Glial fibrillary acidic protein |
| Protein Family: | Glial fibrillary acidic protein |
| UniProt Gene Name: | GFAP |
| UniProt Entry Name: | GFAP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GFAP antibody (CAB14673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (CAB14673) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (CAB14673) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (CAB14673) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-251 MG cells using GFAP Rabbit pAb (CAB14673) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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