Anti-GEN1 Antibody (CAB10001)
- SKU:
- CAB10001
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-GEN1 Antibody |
| 抗体コード: | CAB10001 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 669-908 of human GEN1 (NP_872431.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | H460, MCF-7, Mouse thymus, Mouse spleen |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 669-908 of human GEN1 (NP_872431.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LQDL PLKE RIFT KLSY PQDN LQPD VNLK TLSI LSVK ESCI ANSG SDCT SHLS KDLP GIPL QNES RDSK ILKG DQLL QEDY KVNT SVPY SVSN TVVK TCNV RPPN TALD HSRK VDMQ TTRK ILMK KSVC LDRH SSDE QSAP VFGK AKYT TQRM KHSS QKHN SSHF KESG HNKL SSPK IHIK ETEQ CVRS YETA ENEE SCFP DSTK SSLS SLQC HKKE NNSG TCLD SPLP LRQR LKLR FQST |
| 遺伝子ID: | 348654 |
| Uniprot: | Q17RS7 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 102kDa |
| 観察された分子量: | 103kDa |
| 同義語: | GEN1, Gen |
| バックグラウンド: | This gene encodes a member of the Rad2/xeroderma pigmentosum group G nuclease family, whose members are characterized by N-terminal and internal xeroderma pigmentosum group G nuclease domains followed by helix-hairpin-helix domains and disordered C-terminal domains. The protein encoded by this gene is involved in resolution of Holliday junctions, which are intermediate four-way structures that covalently link DNA during homologous recombination and double-strand break repair. The protein resolves Holliday junctions by creating dual incisions across the junction to produce nicked duplex products that can be ligated. In addition, this protein has been found to localize to centrosomes where it has been implicated in regulation of centrosome integrity. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | GEN1: Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Belongs to the XPG/RAD2 endonuclease family. GEN subfamily. |
| UniProt Protein Details: | Protein type:Hydrolase; EC 3.1.-.- Chromosomal Location of Human Ortholog: 2p24.2 Cellular Component: centrosome; nucleoplasm Molecular Function:crossover junction endodeoxyribonuclease activity; endodeoxyribonuclease activity Biological Process: double-strand break repair via homologous recombination |
| UniProt Code: | Q17RS7 |
| NCBI GenInfo Identifier: | 290457644 |
| NCBI Gene ID: | 348654 |
| NCBI Accession: | Q17RS7.2 |
| UniProt Secondary Accession: | Q17RS7,Q17RS9, Q6ZN37, |
| UniProt Related Accession: | Q17RS7 |
| Molecular Weight: | 102,884 Da |
| NCBI Full Name: | Flap endonuclease GEN homolog 1 |
| NCBI Synonym Full Names: | GEN1, Holliday junction 5' flap endonuclease |
| NCBI Official Symbol: | GEN1 |
| NCBI Official Synonym Symbols: | Gen |
| NCBI Protein Information: | flap endonuclease GEN homolog 1 |
| UniProt Protein Name: | Flap endonuclease GEN homolog 1 |
| Protein Family: | Flap endonuclease |
| UniProt Gene Name: | GEN1 |
| UniProt Entry Name: | GEN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GEN1 antibody (CAB10001) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
View AllClose