Anti-GBP2 Antibody (CAB12994)
- SKU:
- CAB12994
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-GBP2 Antibody |
| 抗体コード: | CAB12994 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 410-520 of human GBP2 (NP_004111.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, A375 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 410-520 of human GBP2 (NP_004111.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QDIF GPLE EDVK QGTF SKPG GYRL FTQK LQEL KNKY YQVP RKGI QAKE VLKK YLES KEDV ADAL LQTD QSLS EKEK AIEV ERIK AESA EAAK KMLE EIQK KNEE MMEQ KEK |
| 遺伝子ID: | 2634 |
| Uniprot: | P32456 |
| セルラーロケーション: | Cytoplasm, Golgi apparatus membrane, Lipid-anchor, Membrane, perinuclear region |
| 計算された分子量: | 67kDa |
| 観察された分子量: | 67kDa/70kDa |
| 同義語: | GBP2 |
| バックグラウンド: | This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas. |
| UniProt Protein Function: | GBP2: a member of the family of large GTP-binding proteins (GBPs) that is inducible by interferon. Possesses a high GTP hydrolysis activity. GBPs are the most abundant cellular proteins expressed in response to interferon-gamma (IFN-gamma). IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) strongly induce the expression of GBP-1, -2, and -3. |
| UniProt Protein Details: | Protein type:Hydrolase; Vesicle Chromosomal Location of Human Ortholog: 1p22.2 Cellular Component: cytosol Molecular Function:protein binding Biological Process: immune response |
| NCBI Summary: | This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas. [provided by RefSeq, Jul 2013] |
| UniProt Code: | P32456 |
| NCBI GenInfo Identifier: | 226694187 |
| NCBI Gene ID: | 2634 |
| NCBI Accession: | P32456.3 |
| UniProt Secondary Accession: | P32456,Q6GPH0, Q6IAU2, Q86TB0, |
| UniProt Related Accession: | P32456 |
| Molecular Weight: | 67,209 Da |
| NCBI Full Name: | Guanylate-binding protein 2 |
| NCBI Synonym Full Names: | guanylate binding protein 2 |
| NCBI Official Symbol: | GBP2 |
| NCBI Protein Information: | guanylate-binding protein 2 |
| UniProt Protein Name: | Guanylate-binding protein 2 |
| UniProt Synonym Protein Names: | GTP-binding protein 2; GBP-2; HuGBP-2; Guanine nucleotide-binding protein 2; Interferon-induced guanylate-binding protein 2 |
| Protein Family: | Guanylate-binding protein |
| UniProt Gene Name: | GBP2 |
| UniProt Entry Name: | GBP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GBP2 antibody (CAB12994) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Western blot analysis of extracts of 293T cells, using GBP2 antibody (CAB12994) at 1:1000 dilution.293T cells treated with different concentrations of IFNA1. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of L929 cells using GBP2 Rabbit pAb (CAB12994) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |