Anti-GAR1 Antibody (CAB12748)
- SKU:
- CAB12748
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GAR1 Antibody |
| 抗体コード: | CAB12748 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-170 of human GAR1 (NP_061856.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse kidney, Rat spleen |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-170 of human GAR1 (NP_061856.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | KGQD QGPP ERVV LLGE FLHP CEDD IVCK CTTD ENKV PYFN APVY LENK EQIG KVDE IFGQ LRDF YFSV KLSE NMKA SSFK KLQK FYID PYKL LPLQ RFLP RPPG EKGP PRG |
| 遺伝子ID: | 54433 |
| Uniprot: | Q9NY12 |
| セルラーロケーション: | Cajal body, Nucleus, nucleolus |
| 計算された分子量: | 20kDa/22kDa |
| 観察された分子量: | 28kDa |
| 同義語: | GAR1, NOLA1 |
| バックグラウンド: | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene. |
| UniProt Protein Function: | NOLA1: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Belongs to the GAR1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nucleolus; RNA processing; RNA-binding Chromosomal Location of Human Ortholog: 4q25 Cellular Component: fibrillar center; nuclear chromosome, telomeric region; nucleoplasm; nucleus; telomerase holoenzyme complex Molecular Function:protein binding; RNA binding Biological Process: rRNA pseudouridine synthesis; snoRNA guided rRNA pseudouridine synthesis; telomere maintenance via telomerase |
| NCBI Summary: | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NY12 |
| NCBI GenInfo Identifier: | 51828015 |
| NCBI Gene ID: | 54433 |
| NCBI Accession: | Q9NY12.1 |
| UniProt Secondary Accession: | Q9NY12,Q5MJQ2, |
| UniProt Related Accession: | Q9NY12 |
| Molecular Weight: | 22kDa |
| NCBI Full Name: | H/ACA ribonucleoprotein complex subunit 1 |
| NCBI Synonym Full Names: | GAR1 ribonucleoprotein |
| NCBI Official Symbol: | GAR1 |
| NCBI Official Synonym Symbols: | NOLA1 |
| NCBI Protein Information: | H/ACA ribonucleoprotein complex subunit 1 |
| UniProt Protein Name: | H/ACA ribonucleoprotein complex subunit 1 |
| UniProt Synonym Protein Names: | Nucleolar protein family A member 1; snoRNP protein GAR1 |
| Protein Family: | D-galacturonate reductase |
| UniProt Gene Name: | GAR1 |
 | Western blot analysis of extracts of various cell lines, using GAR1 antibody (CAB12748) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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