Anti-GABARAPL2 Antibody (CAB7782)
- SKU:
- CAB7782
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| 抗体名: | Anti-GABARAPL2 Antibody |
| 抗体コード: | CAB7782 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL2 (NP_009216.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL2 (NP_009216.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MKWM FKED HSLE HRCV ESAK IRAK YPDR VPVI VEKV SGSQ IVDI DKRK YLVP SDIT VAQF MWII RKRI QLPS EKAI FLFV DKTV PQSS LTMG QLYE KEKD EDGF LYVA YSGE NTFG F |
| 遺伝子ID: | 11345 |
| Uniprot: | P60520 |
| セルラーロケーション: | Cytoplasmic vesicle, Golgi apparatus, autophagosome |
| 計算された分子量: | 13kDa |
| 観察された分子量: | 14kDa |
| 同義語: | GABARAPL2, ATG8, ATG8C, GATE-16, GATE16, GEF-2, GEF2 |
| バックグラウンド: |
| UniProt Protein Function: | GABARAPL2: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Implicated in intra-Golgi transport and post-mitotic Golgi re-assembly. Modulates the activity of SNAREs in the Golgi apparatus and is therefore an essential component of intra-Golgi transport and post-mitotic Golgi re-assembly. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. Phosphorylated upon DNA damage, probably by ATM or ATR. |
| UniProt Protein Details: | Protein type:Vesicle; Microtubule-binding; Ubiquitin-like modifier; Autophagy; Adaptor/scaffold Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: autophagic vacuole; cytoplasm; cytosol; Golgi apparatus; Golgi membrane; intracellular Molecular Function:ATPase binding; beta-tubulin binding; GABA receptor binding; protein binding; SNARE binding; ubiquitin protein ligase binding Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; intra-Golgi vesicle-mediated transport; macroautophagy; mitochondrion degradation; positive regulation of ATPase activity |
| UniProt Code: | P60520 |
| NCBI GenInfo Identifier: | 44888808 |
| NCBI Gene ID: | 11345 |
| NCBI Accession: | P60520.1 |
| UniProt Secondary Accession: | P60520,O08765, Q6FG91, Q9DCP8, Q9UQF7, |
| UniProt Related Accession: | P60520 |
| Molecular Weight: | 13,667 Da |
| NCBI Full Name: | Gamma-aminobutyric acid receptor-associated protein-like 2 |
| NCBI Synonym Full Names: | GABA type A receptor associated protein like 2 |
| NCBI Official Symbol: | GABARAPL2 |
| NCBI Official Synonym Symbols: | ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16 |
| NCBI Protein Information: | gamma-aminobutyric acid receptor-associated protein-like 2 |
| UniProt Protein Name: | Gamma-aminobutyric acid receptor-associated protein-like 2 |
| UniProt Synonym Protein Names: | GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; GATE-16; MAP1 light chain 3-related protein |
| Protein Family: | Gamma-aminobutyric acid receptor-associated protein |
| UniProt Gene Name: | GABARAPL2 |
| UniProt Entry Name: | GBRL2_HUMAN |
 | Western blot analysis of extracts of mouse brain, using GABARAPL2 antibody (CAB7782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GABARAPL2 antibody (CAB7782) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using GABARAPL2 antibody (CAB7782) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of A549 cells using GABARAPL2 antibody (CAB7782). Blue: DAPI for nuclear staining. |
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