Anti-FZR1 Antibody (CAB5550)
- SKU:
- CAB5550
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-FZR1 Antibody |
| 抗体コード: | CAB5550 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 224-493 of human FZR1 (NP_057347.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Mouse heart, Mouse spinal cord |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 224-493 of human FZR1 (NP_057347.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DLSV EGDS VTSV GWSE RGNL VAVG THKG FVQI WDAA AGKK LSML EGHT ARVG ALAW NAEQ LSSG SRDR MILQ RDIR TPPL QSER RLQG HRQE VCGL KWST DHQL LASG GNDN KLLV WNHS SLSP VQQY TEHL AAVK AIAW SPHQ HGLL ASGG GTAD RCIR FWNT LTGQ PLQC IDTG SQVC NLAW SKHA NELV STHG YSQN QILV WKYP SLTQ VAKL TGHS YRVL YLAM SPDG EAIV TGAG DETL RFWN VFSK TRST KESV SVLN LFTR IR |
| 遺伝子ID: | 51343 |
| Uniprot: | Q9UM11 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 44kDa/54kDa/55kDa |
| 観察された分子量: | 50kDa |
| 同義語: | FZR1, CDC20C, CDH1, FZR, FZR2, HCDH, HCDH1 |
| バックグラウンド: |
| UniProt Protein Function: | FZR1: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. The unphosphorylated form interacts with APC/C during mitosis. Interacts with NINL. Interacts (in complex with the anaphase promoting complex APC) with MAD2L2; inhibits FZR1- mediated APC/C activation. Interacts with USP37. Interacts (via WD repeats) with MAK. Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. Belongs to the WD repeat CDC20/Fizzy family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: anaphase-promoting complex; cytosol; nuclear membrane; nucleoplasm Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; G2/M transition DNA damage checkpoint; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle |
| UniProt Code: | Q9UM11 |
| NCBI GenInfo Identifier: | 37537753 |
| NCBI Gene ID: | 51343 |
| NCBI Accession: | Q9UM11.2 |
| UniProt Secondary Accession: | Q9UM11,O75869, Q86U66, Q96NW8, Q9UI96, Q9ULH8, Q9UM10 Q9UNQ1, Q9Y2T8, |
| UniProt Related Accession: | Q9UM11 |
| Molecular Weight: | 44,874 Da |
| NCBI Full Name: | Fizzy-related protein homolog |
| NCBI Synonym Full Names: | fizzy/cell division cycle 20 related 1 |
| NCBI Official Symbol: | FZR1 |
| NCBI Official Synonym Symbols: | FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C |
| NCBI Protein Information: | fizzy-related protein homolog |
| UniProt Protein Name: | Fizzy-related protein homolog |
| UniProt Synonym Protein Names: | CDC20-like protein 1; Cdh1/Hct1 homolog; hCDH1 |
| Protein Family: | Fizzy-related protein |
| UniProt Gene Name: | FZR1 |
| UniProt Entry Name: | FZR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FZR1 antibody (CAB5550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 15s. |
 | Immunofluorescence analysis of HeLa cells using FZR1 antibody (CAB5550). Blue: DAPI for nuclear staining. |
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