Anti-FUT3 Antibody (CAB15058)
- SKU:
- CAB15058
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-FUT3 Antibody |
| 抗体コード: | CAB15058 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FUT3 (NP_000140.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | A-549, HT-29, 293T, LO2 |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FUT3 (NP_000140.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDPL GAAK PQWP WRRC LAAL LFQL LVAV CFFS YLRV SRDD ATGS PRAP SGSS RQDT TPTR PTLL ILLW TWPF HIPV ALSR CSEM VPGT ADCH ITAD RKVY |
| 遺伝子ID: | 2525 |
| Uniprot: | P21217 |
| セルラーロケーション: | Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein |
| 計算された分子量: | 42kDa |
| 観察された分子量: | 50kDa |
| 同義語: | FUT3, CD174, FT3B, FucT-III, LE, Les |
| バックグラウンド: | The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1, 3)-fucosyltransferase and alpha(1, 4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. |
| UniProt Protein Function: | FUT3: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. Belongs to the glycosyltransferase 10 family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase; EC 2.4.1.65 Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: Golgi membrane; membrane Molecular Function:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity Biological Process: biopolymer glycosylation; cell-cell recognition; oligosaccharide biosynthetic process |
| NCBI Summary: | The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P21217 |
| NCBI GenInfo Identifier: | 121137 |
| NCBI Gene ID: | 2525 |
| NCBI Accession: | P21217.1 |
| UniProt Secondary Accession: | P21217,Q32NE7, Q99448, Q99449, B5U7U9, B5U7V0, |
| UniProt Related Accession: | P21217 |
| Molecular Weight: | 42,117 Da |
| NCBI Full Name: | Galactoside 3(4)-L-fucosyltransferase |
| NCBI Synonym Full Names: | fucosyltransferase 3 (Lewis blood group) |
| NCBI Official Symbol: | FUT3 |
| NCBI Official Synonym Symbols: | LE; Les; FT3B; CD174; FucT-III |
| NCBI Protein Information: | galactoside 3(4)-L-fucosyltransferase |
| UniProt Protein Name: | Galactoside 3(4)-L-fucosyltransferase |
| UniProt Synonym Protein Names: | Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III; FucT-III |
| Protein Family: | Fucosyltransferase |
| UniProt Gene Name: | FUT3 |
| UniProt Entry Name: | FUT3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FUT3 antibody (CAB15058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
View AllClose