Anti-FREM2 Antibody (CAB15980)
- SKU:
- CAB15980
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-FREM2 Antibody |
| 抗体コード: | CAB15980 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 47-300 of human FREM2 (NP_997244.4). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, |
| ポジティブサンプル: | Jurkat, HepG2, 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 47-300 of human FREM2 (NP_997244.4). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QPAA FGRA LLSP GLAG AAGV PAEE AIVL ANRG LRVP FGRE VWLD PLHD LVLQ VQPG DRCA VSVL DNDA LAQR PGRL SPKR FPCD FGPG EVRY SHLG ARSP SRDR VRLQ LRYD APGG AVVL PLVL EVEV VFTQ LEVV TRNL PLVV EELL GTSN ALDA RSLE FAFQ PETE ECRV GILS GLGA LPRY GELL HYPQ VPGG AREG GAPE TLLM DCKA FQEL GVRY RHTA ASRS PNRD WIPM VVEL RSRG APVG SPAL KR |
| 遺伝子ID: | 341640 |
| Uniprot: | Q5SZK8 |
| セルラーロケーション: | Cell membrane, Extracellular side, Single-pass type I membrane protein |
| 計算された分子量: | 295kDa/351kDa |
| 観察された分子量: | 351kDa |
| 同義語: | FREM2 |
| バックグラウンド: | This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. |
| UniProt Protein Function: | FREM2: Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. May be required for epidermal adhesion. Defects in FREM2 are a cause of Fraser syndrome (FRASS). Fraser syndrome is a multisystem malformation usually comprising cryptophthalmos, cutaneous syndactyly, ear abnormalities, renal agenesis and congenital heart defects. Belongs to the FRAS1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Extracellular matrix Chromosomal Location of Human Ortholog: 13q13.3 Cellular Component: plasma membrane; integral to membrane; basement membrane Molecular Function:calcium ion binding Biological Process: morphogenesis of an epithelium; cell communication; homophilic cell adhesion; inner ear development Disease: Fraser Syndrome |
| NCBI Summary: | This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. [provided by RefSeq, Apr 2014] |
| UniProt Code: | Q5SZK8 |
| NCBI GenInfo Identifier: | 79749430 |
| NCBI Gene ID: | 341640 |
| NCBI Accession: | NP_997244 |
| UniProt Secondary Accession: | Q5SZK8,Q4QQG1, Q5H9N8, Q5T6Q1, Q6N057, Q6ZSB4, Q7Z305 Q7Z341, |
| UniProt Related Accession: | Q5SZK8 |
| Molecular Weight: | 295,845 Da |
| NCBI Full Name: | FRAS1-related extracellular matrix protein 2 |
| NCBI Synonym Full Names: | FRAS1 related extracellular matrix protein 2 |
| NCBI Official Symbol: | FREM2 |
| NCBI Protein Information: | FRAS1-related extracellular matrix protein 2; ECM3 homolog |
| UniProt Protein Name: | FRAS1-related extracellular matrix protein 2 |
| UniProt Synonym Protein Names: | ECM3 homolog |
| Protein Family: | FRAS1-related extracellular matrix protein |
| UniProt Gene Name: | FREM2 |
| UniProt Entry Name: | FREM2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FREM2 antibody (CAB15980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 150s. |
 | Immunofluorescence analysis of NIH/3T3 cells using FREM2 Rabbit pAb (CAB15980) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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