Anti-FIP200 Antibody (CAB14685)
- SKU:
- CAB14685
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| 抗体名: | Anti-FIP200 Antibody |
| 抗体コード: | CAB14685 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1500 to the C-terminus of human FIP200 (NP_055596.3). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U2OS, HeLa, Mouse testis, Rat testis |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1500 to the C-terminus of human FIP200 (NP_055596.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DFQV GDLV LIIL DERH DNYV LFTV SPTL YFLH SESL PALD LKPG EGAS GASR RPWV LGKV MEKE YCQA KKAQ NRFK VPLG TKFY RVKA VSWN KKV |
| 遺伝子ID: | 9821 |
| Uniprot: | Q8TDY2 |
| セルラーロケーション: | Cytoplasm, Nucleus, Peripheral membrane protein, Preautophagosomal structure membrane, cytosol |
| 計算された分子量: | 182kDa/183kDa |
| 観察された分子量: | 225KDa |
| 同義語: | RB1CC1, ATG17, CC1, FIP200, PPP1R131 |
| バックグラウンド: | The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | RB1CC1: a putative transcription factor that functions as a key regulator of Rb expression. Required for autophagosome formation. Contains a nuclear localization signal, a leucine zipper motif and a coiled-coil structure. Thought to play a biological role in controlling cell growth and progression of various cancers. Probably involved in the tumorigenesis of breast cancer. It is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. Plays a crucial role in muscular differentiation and required for myogenic differentiation. Part of a complex consisting of ATG13, ULK1 and RB1CC1. This complex associates with ATG101. Under starvation conditions, is localized to phagophores. Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells. Expression was difficult to detect in immature proliferating chondroblasts or myogenic cells in embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. Expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. The introduction of the wild-type protein decreased the formation of macroscopic colonies in a cell growth assay. |
| UniProt Protein Details: | Protein type:DNA-binding; Autophagy; Tumor suppressor; Transcription factor Chromosomal Location of Human Ortholog: 8q11 Cellular Component: nuclear membrane; cytoplasm; pre-autophagosomal structure membrane; cytosol Molecular Function:protein binding; protein kinase binding Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell size; heart development; JNK cascade; regulation of autophagy; positive regulation of protein amino acid phosphorylation; liver development; cell cycle; autophagic vacuole formation Disease: Breast Cancer |
| NCBI Summary: | The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2009] |
| UniProt Code: | Q8TDY2 |
| NCBI GenInfo Identifier: | 160359050 |
| NCBI Gene ID: | 9821 |
| NCBI Accession: | Q8TDY2.3 |
| UniProt Related Accession: | Q8TDY2 |
| Molecular Weight: | 182kDa; 183kDa |
| NCBI Full Name: | RB1-inducible coiled-coil protein 1 |
| NCBI Synonym Full Names: | RB1 inducible coiled-coil 1 |
| NCBI Official Symbol: | RB1CC1 |
| NCBI Official Synonym Symbols: | CC1; ATG17; FIP200; PPP1R131 |
| NCBI Protein Information: | RB1-inducible coiled-coil protein 1 |
| UniProt Protein Name: | RB1-inducible coiled-coil protein 1 |
| UniProt Synonym Protein Names: | FAK family kinase-interacting protein of 200 kDa; FIP200 |
| Protein Family: | RB1-inducible coiled-coil protein |
| UniProt Gene Name: | RB1CC1 |
| UniProt Entry Name: | RBCC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FIP200 Rabbit pAb (CAB14685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of various cell lines, using FIP200 Rabbit pAb (CAB14685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using FIP200 Rabbit pAb (CAB14685) at dilution of 1:100 (40x lens). |
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