Anti-FBXW7 Antibody (CAB5872)
- SKU:
- CAB5872
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-FBXW7 Antibody |
| 抗体コード: | CAB5872 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 408-707 of human FBXW7 (NP_361014.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Jurkat, SH-SY5Y, MCF7, mouse testis, rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 408-707 of human FBXW7 (NP_361014.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AVTG KCLR TLVG HTGG VWSS QMRD NIII SGST DRTL KVWN AETG ECIH TLYG HTST VRCM HLHE KRVV SGSR DATL RVWD IETG QCLH VLMG HVAA VRCV QYDG RRVV SGAY DFMV KVWD PETE TCLH TLQG HTNR VYSL QFDG IHVV SGSL DTSI RVWD VETG NCIH TLTG HQSL TSGM ELKD NILV SGNA DSTV KIWD IKTG QCLQ TLQG PNKH QSAV TCLQ FNKN FVIT SSDD GTVK LWDL KTGE FIRN LVTL ESGG SGGV VWRI RASN TKLV CAVG SRNG TEET KLLV LDFD VDMK |
| 遺伝子ID: | 55294 |
| Uniprot: | Q969H0 |
| セルラーロケーション: | Cytoplasm, Nucleus, nucleolus, nucleoplasm |
| 計算された分子量: | 66kDa/70kDa/79kDa |
| 観察された分子量: | 80kDa |
| 同義語: | FBXW7, AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4 |
| バックグラウンド: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Code: | Q969H0 |
| NCBI GenInfo Identifier: | 44887885 |
| NCBI Gene ID: | 55294 |
| NCBI Accession: | Q969H0.1 |
| UniProt Secondary Accession: | Q969H0,Q68DR0, Q96A16, Q96LE0, Q96RI2, Q9NUX6, B7ZLP9 |
| UniProt Related Accession: | Q969H0 |
| Molecular Weight: | 707 |
| NCBI Full Name: | F-box/WD repeat-containing protein 7 |
| NCBI Synonym Full Names: | F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase |
| NCBI Official Symbol: | FBXW7 |
| NCBI Official Synonym Symbols: | AGO; CDC4; FBW6; FBW7; hAgo; FBX30; FBXW6; SEL10; hCdc4; FBXO30; SEL-10 |
| NCBI Protein Information: | F-box/WD repeat-containing protein 7; archipelago; F-box protein FBW7; F-box protein FBX30; F-box protein SEL-10; homolog of C elegans sel-10; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila) |
| UniProt Protein Name: | F-box/WD repeat-containing protein 7 |
| UniProt Synonym Protein Names: | Archipelago homolog; hAgo; F-box and WD-40 domain-containing protein 7; F-box protein FBX30; SEL-10; hCdc4 |
| Protein Family: | Cdc42 |
| UniProt Gene Name: | FBXW7 |
| UniProt Entry Name: | FBXW7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FBXW7 antibody (CAB5872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using FBXW7 antibody (CAB5872) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using FBXW7 antibody (CAB5872) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using FBXW7 antibody (CAB5872) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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