Anti-FBXO25 Antibody (CAB15150)
- SKU:
- CAB15150
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-FBXO25 Antibody |
| 抗体コード: | CAB15150 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 142-291 of human FBXO25 (NP_036305.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | DU145, 293T, Mouse testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 142-291 of human FBXO25 (NP_036305.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | WQQQ LQDL QMTK QVNN GLTL SDLP LHML NNIL YRFS DGWD IITL GQVT PTLY MLSE DRQL WKKL CQYH FAEK QFCR HLIL SEKG HIEW KLMY FALQ KHYP AKEQ YGDT LHFC RHCS ILFW KDSG HPCT AADP DSCF TPVS PQHF IDLF KF |
| 遺伝子ID: | 26260 |
| Uniprot: | Q8TCJ0 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 34kDa/42kDa/43kDa |
| 観察された分子量: | 22kDa |
| 同義語: | FBXO25, FBX25 |
| バックグラウンド: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | FBXO25: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT). A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 8p23.3 Cellular Component: nucleolus; nucleus; SCF ubiquitin ligase complex; ubiquitin ligase complex Molecular Function:actin binding; protein binding; ubiquitin-protein ligase activity Biological Process: protein ubiquitination |
| NCBI Summary: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8TCJ0 |
| NCBI GenInfo Identifier: | 34878757 |
| NCBI Gene ID: | 26260 |
| NCBI Accession: | NP_036305.2 |
| UniProt Secondary Accession: | Q8TCJ0,Q6PJ83, Q7Z4V4, Q9UKB8, |
| UniProt Related Accession: | Q8TCJ0 |
| Molecular Weight: | |
| NCBI Full Name: | F-box only protein 25 isoform 3 |
| NCBI Synonym Full Names: | F-box protein 25 |
| NCBI Official Symbol: | FBXO25 |
| NCBI Official Synonym Symbols: | FBX25 |
| NCBI Protein Information: | F-box only protein 25 |
| UniProt Protein Name: | F-box only protein 25 |
| Protein Family: | F-box only protein |
| UniProt Gene Name: | FBXO25 |
| UniProt Entry Name: | FBX25_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FBXO25 antibody (CAB15150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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