Anti-FANCI Antibody (CAB3447)
- SKU:
- CAB3447
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-FANCI Antibody |
| 抗体コード: | CAB3447 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1061-1328 of human FANCI (NP_001106849.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | HeLa, 293T, MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1061-1328 of human FANCI (NP_001106849.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DQDV EVEK TNHF AIVN LRTA APTV CLLV LSQA EKVL EEVD WLIT KLKG QVSQ ETLS EEAS SQAT LPNQ PVEK AIIM QLGT LLTF FHEL VQTA LPSG SCVD TLLK DLCK MYTT LTAL VRYY LQVC QSSG GIPK NMEK LVKL SGSH LTPL CYSF ISYV QNKS KSLN YTGE KKEK PAAV ATAM ARVL RETK PIPN LIFA IEQY EKFL IHLS KKSK VNLM QHMK LSTS RDFK IKGN ILDM VLRE DGED ENEE GTAS EHGG QNKE PAKK KRKK |
| 遺伝子ID: | 55215 |
| Uniprot: | Q9NVI1 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 27kDa/142kDa/149kDa |
| 観察された分子量: | 149kDa |
| 同義語: | FANCI, KIAA1794 |
| バックグラウンド: | The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group I. Alternative splicing results in two transcript variants encoding different isoforms. |
| UniProt Protein Function: | FANCI: Plays an essential role in the repair of DNA double- strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single- stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage. Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI). A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA repair, damage; Cell cycle regulation Chromosomal Location of Human Ortholog: 15q26.1 Cellular Component: nucleoplasm; membrane; cytoplasm; plasma membrane; nucleus Molecular Function:protein binding; DNA binding Biological Process: positive regulation of protein ubiquitination; cell cycle; DNA repair Disease: Fanconi Anemia, Complementation Group I; Tracheoesophageal Fistula With Or Without Esophageal Atresia |
| NCBI Summary: | The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group I. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NVI1 |
| NCBI GenInfo Identifier: | 212276518 |
| NCBI Gene ID: | 55215 |
| NCBI Accession: | Q9NVI1.4 |
| UniProt Related Accession: | Q9NVI1 |
| Molecular Weight: | ~ 149kDa |
| NCBI Full Name: | Fanconi anemia group I protein |
| NCBI Synonym Full Names: | FA complementation group I |
| NCBI Official Symbol: | FANCI |
| NCBI Official Synonym Symbols: | KIAA1794 |
| NCBI Protein Information: | Fanconi anemia group I protein |
| UniProt Protein Name: | Fanconi anemia group I protein |
| Protein Family: | Fanconi anemia group I protein |
| UniProt Gene Name: | FANCI |
| UniProt Entry Name: | FANCI_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FANCI antibody (CAB3447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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