Anti-ETFB Antibody (CAB16043)
- SKU:
- CAB16043
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ETFB Antibody |
| 抗体コード: | CAB16043 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-230 of human ETFB (NP_001976.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, A-549, 293T, BxPC-3, Mouse brain, Mouse heart, Mouse kidney, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-230 of human ETFB (NP_001976.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AMGA DRGI HVEV PPAE AERL GPLQ VARV LAKL AEKE KVDL VLLG KQAI DDDC NQTG QMTA GFLD WPQG TFAS QVTL EGDK LKVE REID GGLE TLRL KLPA VVTA DLRL NEPR YATL PNIM KAKK KKIE VIKP GDLG VDLT SKLS VISV EDP |
| 遺伝子ID: | 2109 |
| Uniprot: | P38117 |
| セルラーロケーション: | Mitochondrion matrix |
| 計算された分子量: | 27kDa/37kDa |
| 観察された分子量: | 30kDa |
| 同義語: | ETFB, FP585, MADD |
| バックグラウンド: | This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | ETFB: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B). GA2B is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Belongs to the ETF beta-subunit/FixA family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Oxidoreductase Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: intracellular membrane-bound organelle; mitochondrion; mitochondrial matrix Molecular Function:electron carrier activity Biological Process: cellular metabolic process Disease: Multiple Acyl-coa Dehydrogenase Deficiency |
| NCBI Summary: | This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P38117 |
| NCBI GenInfo Identifier: | 62420877 |
| NCBI Gene ID: | 2109 |
| NCBI Accession: | NP_001014763.1 |
| UniProt Secondary Accession: | P38117,Q6IBH7, Q71RF6, Q9Y3S7, B3KNY2, |
| UniProt Related Accession: | P38117 |
| Molecular Weight: | 37,566 Da |
| NCBI Full Name: | electron transfer flavoprotein subunit beta isoform 2 |
| NCBI Synonym Full Names: | electron-transfer-flavoprotein, beta polypeptide |
| NCBI Official Symbol: | ETFB |
| NCBI Official Synonym Symbols: | MADD; FP585 |
| NCBI Protein Information: | electron transfer flavoprotein subunit beta; beta-ETF; electron transfer flavoprotein beta subunit; electron transfer flavoprotein beta-subunit; electron transfer flavoprotein, beta polypeptide; electron-transferring-flavoprotein, beta polypeptide |
| UniProt Protein Name: | Electron transfer flavoprotein subunit beta |
| Protein Family: | Electron transfer flavoprotein |
| UniProt Gene Name: | ETFB |
| UniProt Entry Name: | ETFB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ETFB antibody (CAB16043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of C6 cells using ETFB Polyclonal Antibody (CAB16043) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using ETFB Polyclonal Antibody (CAB16043) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using ETFB Polyclonal Antibody (CAB16043) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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