Anti-ETFA Antibody (CAB7670)
- SKU:
- CAB7670
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ETFA Antibody |
| 抗体コード: | CAB7670 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, A-549, SW620, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MFRA AAPG QLRR AASL LRFQ STLV IAEH ANDS LAPI TLNT ITAA TRLG GEVS CLVA GTKC DKVA QDLC KVAG IAKV LVAQ HDVY KGLL PEEL TPLI LATQ KQFN YTHI CAGA SAFG KNLL PRVA AKLE VAPI SDII AIKS PDTF VRTI YAGN ALCT VKCD EKVK VFSV RGTS FDAA ATSG GSAS SEKA SSTS PVEI SEWL DQKL TKSD RPEL TGAK VVVS GGRG LKSG ENFK LLYD LADQ LHAA VGAS RAAV DAGF VPND MQVG QTGK IVAP ELYI AVGI SGAI QHLA GMKD SKTI VAIN KDPE APIF QVAD YGIV ADLF KVVP EMTE ILKK K |
| 遺伝子ID: | 2108 |
| Uniprot: | P13804 |
| セルラーロケーション: | Mitochondrion matrix |
| 計算された分子量: | 30kDa/35kDa |
| 観察された分子量: | 35kDa |
| 同義語: | ETFA, EMA, GA2, MADD |
| バックグラウンド: | ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ETFA: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Defects in ETFA are the cause of glutaric aciduria type 2A (GA2A); also known as glutaricaciduria IIA. GA2A is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Belongs to the ETF alpha-subunit/FixB family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 15q23-q25 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:acyl-CoA binding; acyl-CoA dehydrogenase activity; electron carrier activity; FAD binding; oxidoreductase activity Biological Process: fatty acid beta-oxidation using acyl-CoA dehydrogenase; lipid homeostasis Disease: Multiple Acyl-coa Dehydrogenase Deficiency |
| NCBI Summary: | ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P13804 |
| NCBI GenInfo Identifier: | 119636 |
| NCBI Gene ID: | 2108 |
| NCBI Accession: | P13804.1 |
| UniProt Secondary Accession: | P13804,Q53XN3, B4DT43, |
| UniProt Related Accession: | P13804 |
| Molecular Weight: | 30,026 Da |
| NCBI Full Name: | Electron transfer flavoprotein subunit alpha, mitochondrial |
| NCBI Synonym Full Names: | electron transfer flavoprotein alpha subunit |
| NCBI Official Symbol: | ETFA |
| NCBI Official Synonym Symbols: | EMA; GA2; MADD |
| NCBI Protein Information: | electron transfer flavoprotein subunit alpha, mitochondrial |
| UniProt Protein Name: | Electron transfer flavoprotein subunit alpha, mitochondrial |
| Protein Family: | Electron transfer flavoprotein |
| UniProt Gene Name: | ETFA |
| UniProt Entry Name: | ETFA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ETFA antibody (CAB7670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using ETFA Rabbit pAb (CAB7670) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using ETFA Rabbit pAb (CAB7670) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using ETFA Rabbit pAb (CAB7670) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human thyroid cancer using ETFA Rabbit pAb (CAB7670) at dilution of 1:200 (40x lens). |
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